Trehalose pretreatment induces salt tolerance in rice (Oryza sativa L.) seedlings: oxidative damage and co-induction of antioxidant defense and glyoxalase systems

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• 作者：Mohammad Golam Mostofa ; Mohammad Anwar Hossain ; Masayuki Fujita
• 关键词：Salt toxicity ; Osmoprotectants ; Oxidative stress ; Trehalose ; Antioxidant defense ; Glyoxalase system ; Oryza sativa L.
• 刊名：Protoplasma
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：252
• 期：2
• 页码：461-475
• 全文大小：1,216 KB
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  16. Elia AC,
• 作者单位：Mohammad Golam Mostofa (1) (2)
  Mohammad Anwar Hossain (3)
  Masayuki Fujita (1)
  
  1. Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795, Japan
  2. Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh
  3. Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Zoology
  
• 出版者：Springer Wien
• ISSN：1615-6102

文摘

Salinity in the form of abiotic stress adversely effects plant growth, development, and productivity. Various osmoprotectants are involved in regulating plant responses to salinity; however, the precise role of trehalose (Tre) in this process remains to be further elucidated. The present study investigated the regulatory role of Tre in alleviating salt-induced oxidative stress in hydroponically grown rice seedlings. Salt stress (150 and 250?mM NaCl) for 72?h resulted in toxicity symptoms such as stunted growth, severe yellowing, and leaf rolling, particularly at 250?mM NaCl. Histochemical observation of reactive oxygen species (ROS; O2 ?? and H2O2) indicated evident oxidative stress in salt-stressed seedlings. In these seedlings, the levels of lipoxygenase (LOX) activity, malondialdehyde (MDA), H2O2, and proline (Pro) increased significantly whereas total chlorophyll (Chl) and relative water content (RWC) decreased. Salt stress caused an imbalance in non-enzymatic antioxidants, i.e., ascorbic acid (AsA) content, AsA/DHA ratio, and GSH/GSSG ratio decreased but glutathione (GSH) content increased significantly. In contrast, Tre pretreatment (10?mM, 48?h) significantly addressed salt-induced toxicity symptoms and dramatically depressed LOX activity, ROS, MDA, and Pro accumulation whereas AsA, GSH, RWC, Chl contents, and redox status improved considerably. Salt stress stimulated the activities of SOD, GPX, APX, MDHAR, DHAR, and GR but decreased the activities of CAT and GST. However, Tre-pretreated salt-stressed seedlings counteracted SOD and MDHAR activities, elevated CAT and GST activities, further enhanced APX and DHAR activities, and maintained GPX and GR activities similar to the seedlings stressed with salt alone. In addition, Tre pretreatment enhanced the activities of methylglyoxal?detoxifying enzymes (Gly I and Gly II) more efficiently in salt-stressed seedlings. Our results suggest a role for Tre in protecting against salt-induced oxidative damage attributed to reduced ROS accumulation, elevation of non-enzymatic antioxidants, and co-activation of the antioxidative and glyoxalase systems.