Optimization of the culture condition for an antitumor bacterium Serratia proteamacula 657 and identification of the active compounds

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• 作者：Li Miao (1) (2)
  Xueling Wang (1)
  Wei Jiang (1)
  Shengping Yang (2)
  Huiru Zhou (1)
  Youpeng Zhai (1)
  Xiaojian Zhou (1)
  Kunming Dong (1)
  
• 关键词：Antitumor activity ; Serratia proteamacula ; Optimization of culture conditions ; Prodigiosin
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：29
• 期：5
• 页码：855-863
• 全文大小：438KB
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• 作者单位：Li Miao (1) (2)
  Xueling Wang (1)
  Wei Jiang (1)
  Shengping Yang (2)
  Huiru Zhou (1)
  Youpeng Zhai (1)
  Xiaojian Zhou (1)
  Kunming Dong (1)
  
  1. School of Environmental Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China
  2. Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China
  
• ISSN：1573-0972

文摘

Exploration of novel active anti-tumor compounds from marine microbes for pharmaceutical applications has been a continuously hot spot in natural product research. Bacterial growth and metabolites may greatly vary under different culture conditions. In this study, the effects of different culture conditions and medium components on the growth and bioactive metabolites of Serratia proteamacula 657, an anti-tumor bacterium found in our previous study, were investigated. The results showed that lower temperature, weak acidic condition and solid fermentation favored the bacterial growth and the production of active compounds. Four components in the culture medium, NaCl, peptone, yeast extract and MgSO4, were found important to the bacterial growth and active compounds production in medium optimization. Under the optimized condition of solid state fermentation at pH 6.0-.0, 23-5?°C, with the MgSO4-free medium containing 10.0?g/L peptone, 1.0?g/L yeast extract and 19.45?g/L NaCl, the antitumor activity of S. proteamacula 657 and the yield of crude extracts increased about 15 times and 6 times than the sample obtained in the original liquid fermentation, respectively. The active components in the metabolites of S. proteamacula 657 were identified as a homolog of prodigiosin, a red bacterial pigment, based on the analysis of the NMR and GC–MS. The bacterium S. proteamacula 657, which is adapted to lower temperature, produced prodigiosin-like pigments with highly antitumor activity, suggesting the bacterium is a potential new source for prodigiosin production.