The effects of IGF1 on the melanogenesis in alpaca melanocytes in vitro

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• 作者：Shuaipeng Hu ; Yu Liu ; Shanshan Yang…
• 关键词：IGF1 ; Melanogenesis ; Melanocyte ; Alpaca
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：52
• 期：8
• 页码：806-811
• 全文大小：943 KB
• 刊物主题：Cell Biology; Developmental Biology; Stem Cells; Cell Culture; Animal Genetics and Genomics;
• 出版者：Springer US
• ISSN：1543-706X
• 卷排序：52

文摘

In order to investigate the effects of the insulin-like growth factor 1(IGF-1) on alpaca melanocyte in vitro, different dosees of IGF1 (0, 10, 20, 40 ng/ml) were added in the medium of alpaca melanocyte. The RTCA machine was used to monitor the proliferation, quantitative real-time PCR, and western blot to test the relative gene expression, ELISA to test cAMP production, and spectrum method to test the melanin production. The results showed that compared to the normal melanocyte, the proliferation of melanocytes was increased within 60 h following adding IGF1. It also showed that cAMP content produced by melanocytes was increased, microphthalmia-associtated transcription factor (MITF), tyrosinase (TYR) and tyrosinase-related protein 2 (TYRP2) expression was increased, and melanin production with most obvious change in 10 ng/ml supplementary group, when compared with the control group. The results suggested that IGF1 with the dose of 10 ng/ml had the important effects on the melanogenesis in alpaca melanocyte by the cAMP pathway.