RELIC: a novel dye-bias correction method for Illumina Methylation BeadChip

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• 作者：Zongli Xu ; Sabine A. S. Langie ; Patrick De Boever ; Jack A. Taylor…
• 关键词：RELIC ; DNA methylation ; Dye ; bias correction ; Illumina HumanMethylation450 ; Illumina MethylationEPIC ; Data preprocessing
• 刊名：BMC Genomics
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：18
• 期：1
• 全文大小：752KB
• 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
• 出版者：BioMed Central
• ISSN：1471-2164
• 卷排序：18

文摘

BackgroundThe Illumina Infinium HumanMethylation450 BeadChip and its successor, Infinium MethylationEPIC BeadChip, have been extensively utilized in epigenome-wide association studies. Both arrays use two fluorescent dyes (Cy3-green/Cy5-red) to measure methylation level at CpG sites. However, performance difference between dyes can result in biased estimates of methylation levels.