Anti-GAPDHS antibodies: a biomarker of immune infertility

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• 作者：Jun Fu ; Rongyan Yao ; Yanyun Luo ; Dantong Yang ; Yang Cao ; Yi Qiu…
• 关键词：Immune infertility ; GAPDHS ; ELISA ; Antisperm antibodies ; Primary infertility
• 刊名：Cell and Tissue Research
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：364
• 期：1
• 页码：199-207
• 全文大小：609 KB
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• 作者单位：Jun Fu (1)
  Rongyan Yao (1)
  Yanyun Luo (1)
  Dantong Yang (2)
  Yang Cao (3)
  Yi Qiu (2)
  Wei Song (1)
  Shiying Miao (1)
  Yiqun Gu (3)
  Linfang Wang (1)
  
  1. State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, School of Basic Medicine Peking Union Medical College, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, No.5 Dong Dan San Tiao, Dongcheng, Beijing, People’s Republic of China
  2. Key Laboratory of Birth Regulation and Control Technology of National Health and Family Planning Commission of China, Shandong Provincial Key Laboratory for Improving Birth Outcome Technique, Shandong Provincial Family Planning Institute of Science and Technology, Jinan, People’s Republic of China
  3. National Health and Family Planning Key Laboratory of Male Reproductive Health, National Research Institute for Family Planning, World Health Organization Collaboration Centre for Research in Human Reproduction, Beijing, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

Numerous investigations have focused on the detection of antisperm antibodies, which have a naturally occurring impact on male and female fertility. In this study, spermatogenic glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) was considered to be a candidate biomarker of immune infertility. The concentrations of anti–GAPDHS antibodies in the sera of sterile individuals and fertile couples were measured by enzyme-linked immunosorbent assay. Sera were collected from immune infertile (n = 175) and fertile (n = 237) individuals and were screened by tray agglutination tests (TAT). Infertile sera were further divided into two groups according to the serum titers obtained by TAT (titers ≤ 1:8, n = 58; titers > 1:8, n = 117). The concentrations of anti–GAPDHS antibodies were significantly higher in the immune infertile group than in the fertile group and were much higher with regard to the increased degrees of sperm agglutination (titers > 1:8). Surprisingly, we found statistically significantly higher concentrations of antibodies in the sera of infertile men than in those of infertile women, and a similar statistical result was obtained in the sera when primary infertility was compared with secondary infertility. Thus, anti–GAPDHS antibodies seem to be a sensitive parameter in immune infertile detection and might be one of the main factors causing immune infertility. This factor might be valuable as an indicator in the clinical diagnosis and monitoring treatment of infertility.