Production of bioactive polysaccharides by Inonotus obliquus under submerged fermentation supplemented with lignocellulosic biomass and their antioxidant activity
详细信息 查看全文
- 作者：Xiangqun Xu (1)
Yan Hu (1)
Lili Quan (1) - 关键词：Biodegradation ; Inonotus obliquus ; Lignocellulosic biomass ; Polysaccharides ; Submerged fermentation
- 刊名：Bioprocess and Biosystems Engineering
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：37
- 期：12
- 页码：2483-2492
- 全文大小：459 KB
- 参考文献：1. Zhong JJ, Xiao JH (2009) Secondary metabolites from higher fungi: discovery, bioactivity, and bioproduction. Adv Biochem Eng Biot 113:79-50
2. Zheng W, Miao K, Liu Y, Zhao Y, Zhang M, Pan S, Dai Y (2010) Chemical diversity of biologically active metabolites in the sclerotia of / Inonotus obliquus and submerged culture strategies for up-regulating their production. Appl Microbiol Biotechnol 87:1237-254 CrossRef
3. Ju HK, Chung HW, Hong SS, Park JH, Lee J, Kwon SW (2010) Effect of steam treatment on soluble phenolic content and antioxidant activity of the Chaga mushroom ( / Inonotus obliquus). Food Chem 119:619-25 CrossRef
4. Kim YO, Park HW, Kim JH, Lee JY, Moon SH, Shin CS (2006) Anti-cancer effect and structural characterization of endo-polysaccharide from cultivated mycelia of / Inonotus obliquus. Life Sci 79:72-0 CrossRef
5. Zhang LX, Fan C, Liu SC, Zang ZF, Jiao LL, Zhang LP (2011) Chemical composition and antitumor activity of polysaccharide from / Inonotus obliquus. J Med Plants Res 5:1251-260
6. Fan LP, Ding SD, Ai LZ, Deng KQ (2012) Antitumor and immunomodulatory activity of water-soluble polysaccharide from / Inonotus obliquus. Carbohyd Polym 90:870-74 CrossRef
7. Kim YO, Han SB, Lee HW, Ahn HJ, Yoon YD, Jung JK, Kim HM, Shin CS (2005) Immunostimulating effect of the endo-polysaccharide produced by submerged culture of / Inonotus obliquus. Life Sci 77:2438-456 CrossRef
8. Won DP, Lee JS, Kwon DS, Lee KE, Shin WC, Hong EK (2011) Immunostimulating activity by polysaccharides isolated from fruiting body of / Inonotus obliquus. Mol Cells 31:165-73 CrossRef
9. Xu XQ, Wu YD, Chen H (2011) Comparative antioxidative characteristics of polysaccharide-enriched extracts from natural sclerotia and cultured mycelia in submerged fermentation of / Inonotus obliquus. Food Chem 127:74-9 CrossRef
10. Xu XQ, Zhu JW (2011) Enhanced phenolic antioxidants production in submerged cultures of / Inonotus obliquus in a ground corn stover medium. Biochem Eng J 58-9:103-09 CrossRef
11. Zhu LH, Xu XQ (2013) Stimulatory effect of different lignocellulosic materials for phenolic compound production and antioxidant activity from / Inonotus obliquus in submerged fermentation. Appl Biochem Biotechnol 169:2138-152 CrossRef
12. Chen H, Yan MC, Zhu JW, Xu XQ (2011) Enhancement of exo-polysaccharide production and antioxidant activity in submerged cultures of / Inonotus obliquus by lignocellulose decomposition. J Ind Microbiol Biotechnol 38:291-98 CrossRef
13. Xiang YL, Xu XQ, Li J (2012) Chemical properties and antioxidant activity of exopolysaccharides fractions from mycelial culture of / Inonotus obliquus in a ground corn stover medium. Food Chem 134:1899-905 CrossRef
14. Yan Q, Han L, Jiang ZQ (2004) Removal of protein from the crude astragalus polysaccharides by proteases. Food Sci Technol 6:23-6
15. Miller G (1959) Use of dinitrosalicylic reagent for the determination of reducing sugar. Anal Chem 31:426-28 CrossRef
16. Dubois M, Gilles KA, Hamilton J, Rebers PA, Smith F (1956) Colorimetric method for determination of sugar and relative substances. Anal Chem 28:350-66 CrossRef
17. Chaplin MF, Kennedy JF (1994) Carbohydrate analysis: a practical approach. Oxford University Press, Oxford
18. Bradford MM (1976) A rapid sensitive method for the quantitation of microgram - 作者单位：Xiangqun Xu (1)
Yan Hu (1)
Lili Quan (1)
1. Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou, 310018, China - ISSN：1615-7605
文摘
The effect of lignocellulose degradation in wheat straw, rice straw, and sugarcane bagasse on the accumulation and antioxidant activity of extra- (EPS) and intracellular polysaccharides (IPS) of Inonotus obliquus under submerged fermentation were first evaluated. The wheat straw, rice straw, and sugarcane bagasse increased the EPS accumulation by 91.4, 78.6, and 74.3?% compared with control, respectively. The EPS and IPS extracts from the three lignocellulose media had significantly higher hydroxyl radical- and 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity than those from the control medium. Of the three materials, wheat straw was the most effective lignocellulose in enhancing the mycelia growth, accumulation and antioxidant activity of I. obliquus polysaccharides (PS). The carbohydrate and protein content, as well as the monosaccharide compositions of the EPS and IPS extracts, were correlated with sugar compositions and dynamic contents during fermentation of individual lignocellulosic materials. The enhanced accumulation of bioactive PS of cultured I. obliquus supplemented with rice straw, wheat straw, and bagasse was evident.