Structure of intergenic spacer IGS1 of ribosomal operon from Schistidium mosses

详细信息    查看全文

• 作者：I. A. Milyutina ; E. A. Ignatova ; M. S. Ignatov ; D. V. Goryunov…
• 关键词：rDNA ; IGS ; intergenic spacer ; conserved motifs ; mosses
• 刊名：Biochemistry (Moscow)
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：80
• 期：11
• 页码：1485-1491
• 全文大小：412 KB
• 参考文献：1.Wicke, S., Costa, A., Muñoz, J., and Quandt, D. (2010) Restless 5S: the rearrangement(s) and evolution of the nuclear ribosomal DNA in land plants, Mol. Phylogenet. Evol., 61, 321–332.CrossRef
  2.Rocha, P. S., and Bertrand, H. (1995) Structure and comparative analysis of the rDNA intergenic spacer of Brassica rapa, Eur. J. Biochem., 229, 550–557.CrossRef PubMed
  3.Delcasso-Tremousaygue, D., Grellet, F., Panabieres, F., Ananiev, E. D., and Delseny, M. (1988) Structural and transcriptional characterization of the external spacer of a ribosomal RNA nuclear gene from a higher plant, Eur. J. Biochem., 172, 767–776.CrossRef PubMed
  4.Doelling, J. H., and Pikaard, C. S. (1995) The minimal ribosomal RNA gene promoter of Arabidopsis thaliana includes a critical element at the transcription initiation site, Plant J., 8, 683–692.CrossRef PubMed
  5.Doelling, J. H., Gaudino, R. J., and Pikaard, C. S. (1993) Functional analysis of Arabidopsis thaliana rRNA gene and spacer promoters in vivo and by transient expression, Proc. Natl. Acad. Sci. USA, 90, 7528–7532.PubMedCentral CrossRef PubMed
  6.Castiglione, M. R., Gelati, M. T., Cremonini, R., and Frediani, M. (2013) The intergenic spacer region of the rDNA in Haplopappus gracilis (Nutt.) Gray, Protoplasma, 250, 683–689.CrossRef
  7.Garcia, S., Panero, J. L., Siroky, J., and Kovarik, A. (2010) Repeated reunions and splits feature the highly dynamic evolution of 5S and 35S ribosomal RNA genes (rDNA) in the Asteraceae family, BMC Plant Biol., 10, 176.PubMedCentral CrossRef PubMed
  8.Milyutina, I. A., Goryunov, D. V., Ignatov, M. S., Ignatova, E. A., and Troitsky, A. V. (2010) The phylogeny of Schistidium (Bryophyta, Grimmiaceae) based on the primary and secondary structure of nuclear rDNA internal transcribed spacers, Mol. Biol. (Moscow), 44, 883–897.CrossRef
  9.Milyutina, I. A., and Ignatov, M. S. (2015) Conserved motifs in the primary and secondary ITS1 structures of bryophytes, Mol. Biol. (Moscow), 49, 348–357.CrossRef
  10.Fukunaga, K., Ichitani, K., Taura, S., Sato, M., and Kawase, M. (2005) Ribosomal DNA intergenic spacer sequence in foxtail millet, Setaria italica (L.) P. Beauv. and its characterization and application to typing of foxtail millet landraces, Hereditas, 142, 38–44.CrossRef PubMed
  11.Capesius, I. (1997) Analysis of the ribosomal RNA gene repeat from the moss Funaria hygrometrica, Plant Mol. Biol., 33, 559–564.CrossRef PubMed
  12.Grummt, I., Maier, U., Ohrlein, A., Hassouna, N., and Bachellerie, J. P. (1985) Transcription of mouse rDNA terminates downstream of the 3'-end of 28S RNA and involves interaction of factors with repeated sequences in the 3' spacer, Cell, 43, 801–810.CrossRef PubMed
  13.Abreu-Blanco, M. T., Ramirez, J. L., Pinto-Santini, D. M., Papadopoulou, B., and Guevara, P. (2010) Analysis of ribosomal RNA transcription termination and 3'-end processing in Leishmania amazonensis, Gene, 451, 15–22.CrossRef PubMed
  
• 作者单位：I. A. Milyutina (1)
  E. A. Ignatova (2)
  M. S. Ignatov (3)
  D. V. Goryunov (1)
  A. V. Troitsky (1)
  
  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia
  2. Biological Faculty, Lomonosov Moscow State University, 119991, Moscow, Russia
  3. Main Botanical Garden, Russian Academy of Sciences, 127276, Moscow, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Microbiology
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3040

文摘

The structure of the intergenic spacer 1 (IGS1) of the ribosomal operon from 12 species of Schistidium mosses was studied. In the IGS1 sequences of these species, three conserved regions and two areas of GCand A-enriched repeats were identified. All of the studied mosses have a conserved pyrimidine-enriched motif at the 5′-end of IGS1. Species-specific nucleotide substitutions and insertions were found in the conserved areas. The repeated units contain single nucleotide substitutions that make unique the majority of repeated units. The positions of such repeats in IGS1 are species-specific, but their number can vary within the species and among operons of the same specimen. The comparison of IGS1 sequences from the Schistidium species and from representatives of ten other moss genera revealed the presence of common conserved motifs with similar localization. Presumably, these motifs are elements of termination of the pre-rRNA transcription and processing of rRNA.