UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection

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• 作者：Aldino Viegas ; Thibault Viennet ; Tsyr-Yan Yu ; Frank Schumann…
• 关键词：Interleaved acquisition ; Low ; γ nuclei ; UTOPIA ; NMR ; 13C ; detection
• 刊名：Journal of Biomolecular NMR
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：64
• 期：1
• 页码：9-15
• 全文大小：1,149 KB
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• 作者单位：Aldino Viegas (1)
  Thibault Viennet (1) (2)
  Tsyr-Yan Yu (3) (6)
  Frank Schumann (5)
  Wolfgang Bermel (4)
  Gerhard Wagner (3)
  Manuel Etzkorn (1) (2)
  
  1. Institute of Physical Biology, Heinrich-Heine-University, Düsseldorf, Germany
  2. Instititue of Complex Systems, Forschungszentrum Jülich, Jülich, Germany
  3. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
  6. Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan
  5. Bruker BioSpin GmbH, Fällanden, Switzerland
  4. Bruker BioSpin GmbH, Rheinstetten, Germany
  
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Biophysics and Biomedical Physics
  Polymer Sciences
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-5001

文摘

A growing number of nuclear magnetic resonance (NMR) spectroscopic studies are impaired by the limited information content provided by the standard set of experiments conventionally recorded. This is particularly true for studies of challenging biological systems including large, unstructured, membrane-embedded and/or paramagnetic proteins. Here we introduce the concept of unified time-optimized interleaved acquisition NMR (UTOPIA-NMR) for the unified acquisition of standard high-γ (e.g. 1H) and low-γ (e.g. 13C) detected experiments using a single receiver. Our aim is to activate the high level of polarization and information content distributed on low-γ nuclei without disturbing conventional magnetization transfer pathways. We show that using UTOPIA-NMR we are able to recover nearly all of the normally non-used magnetization without disturbing the standard experiments. In other words, additional spectra, that can significantly increase the NMR insights, are obtained for free. While we anticipate a broad range of possible applications we demonstrate for the soluble protein Bcl-x_L (ca. 21 kDa) and for OmpX in nanodiscs (ca. 160 kDa) that UTOPIA-NMR is particularly useful for challenging protein systems including perdeuterated (membrane) proteins.