Characterization of eight cytosolic sHSP genes and their expression in Capsella bursa-pastoris

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• 作者：P. Tao (1)
  L. Liu (1)
  J. B. Wang (1) jbwang@whu.edu.cn
• 关键词：Additional key words Arabidopsis thaliana – Capsella rubella – heat shock – qRT ; PCR – RACE – 3′ ; UTR
• 刊名：Biologia Plantarum
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：56
• 期：4
• 页码：648-656
• 全文大小：366.0 KB
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• 作者单位：1. Key Laboratory of the MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, 430072 P.R. China
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1573-8264

文摘

Small heat shock proteins (sHSPs) are crucial components of the plant response to heat shock. We identified and analyzed eight sHSP genes of Capsella bursa-pastoris to better understand the ability of this species to adapt. Eight genes were initially cloned and sequenced from the mature embryo cDNA pool. They belong to the cytosolic I (CI), cytosolic II (CII), and cytosolic III (CIII) subfamilies. One CI sHSP gene was homologous to that of C. rubella. Sequence analysis using 3′ RACE revealed that there are two or more variable 3′-untranslated regions (UTRs) in these sHSP transcripts. The transcriptional levels of the eight sHSP genes were analyzed in different organs and developmental stages via qRT-PCR. Eight genes were significantly up-regulated in young leaves exposed to heat stress at 42 °C, and also showed differential responses to ABA treatment. We also compared expression of these genes with corresponding Arabidopsis sHSP genes and found some differences between the two species.