Genetic Changes Following Hybridization and Genome Doubling in Synthetic Brassica napus

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• 作者：Yanhao Xu (12)
  Hong Xu (2)
  Xiaoming Wu (3)
  Xiaoping Fang (3)
  Jianbo Wang (2) jbwang@whu.edu.cn
• 关键词：Hybridization – ; Genome doubling – ; AFLP analysis – ; Brassica napus
• 刊名：Biochemical Genetics
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：50
• 期：7-8
• 页码：616-624
• 全文大小：224.8 KB
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• 作者单位：1. Engineering Research Center of Wetland Agriculture in the Middle Reaches of the Yangtze River, Ministry of Education, College of Agriculture, Yangtze University, Jingzhou, 434025 Hubei, China2. Key Laboratory of the MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, 430072 China3. Key Laboratory of Oil Crops Genetic Improvement of the Ministry of Agriculture, Oil Crops Research Institute, CAAS, Wuhan, 430062 China
• ISSN：1573-4927

文摘

Genetic changes were investigated in two sets of independently synthesized Brasscia napus allopolyploids by the AFLP approach in the present study. We found that 1.17 % of the loci showed genetic changes following both hybridization and genome doubling in the synthesized B. napus F04J2 relative to its diploid progenitors, B. rapa (AA genome) and B. oleracea (CC genome). No significant difference between the proportion of A-genome-specific genetic changes and that of C-genome-specific genetic changes was detected in B. napus F04J2. Approximately 0.6 % of the loci displayed genetic changes following somatic genome doubling in the amphidiploid B. napus DCE11 relative to the amphihaploid in the dimorphic plants. This study showed that rapid genetic changes occurred after hybridization and/or genome doubling in synthesized B. napus allopolyploids and indicated that both hybridization and genome doubling could affect the genomic architecture in newly formed allopolyploids.