Multilocus sequence typing analysis of Cronobacter spp. isolated from China

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• 作者：Jing-Hua Cui ; Xiao-Li Du ; Rong-Jie Wei ; Hai-Jian Zhou ; Wei Li…
• 关键词：Cronobacter spp. ; Multilocus sequence typing (MLST) ; Sequence type (ST) ; Clonal complexes
• 刊名：Archives of Microbiology
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：197
• 期：5
• 页码：665-672
• 全文大小：546 KB
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• 作者单位：Jing-Hua Cui (1)
  Xiao-Li Du (1)
  Rong-Jie Wei (2)
  Hai-Jian Zhou (1)
  Wei Li (1)
  Stephen Forsythe (3)
  Zhi-Gang Cui (1)
  
  1. National Institute for Communicable Disease Control and Prevention and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China
  2. Qinghai Institute for Endemic Diseases Prevention and Control, Xining, China
  3. Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, UK
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Microbial Ecology
  Biochemistry
  Cell Biology
  Biotechnology
  Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-072X

文摘

Multilocus sequence typing (MLST) has proven to be an effective approach for the subtyping isolates of the Cronobacter genus and to exhibit a high level of discrimination between isolates. In this study, 151 Cronobacter strains were isolated from different sources and provinces across China from 2010 to 2012 and analyzed by MLST. Their sequence type profiles were compared with strains from other countries which were widely geographically and temporally distributed. Out of 151 strains in this study, the majority of strains were Cronobacter sakazakii (70.9?%), C. malonaticus (15.9?%), C. dublinensis (10.6?%), C. turicensis (2.0?%), and C. muytjensii (0.7?%). The strains were divided into 85 sequence types (STs), among which only 17 had previously been reported in other countries. The 85 identified STs for the Cronobacter genus were grouped into 14 clonal complexes and 47 singletons according to eBURST algorithm. The Cronobacter isolated from China showed a high diversity when they were subtyped using the MLST method. When compared to the Cronobacter PubMLST database, some sequence types of strains cultured from food and/or water in this study were also the same with strains isolated from patients in other countries as reported previously. This result showed the potential hazard of strains contaminating water and weaning food from China.