Mechanistic Aspects of the Photodynamic Inactivation of Vancomycin-Resistant Enterococci Mediated by 5-Aminolevulinic Acid and 5-Aminolevulinic Acid Methyl Ester
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- 作者：Chengcheng Liu ; Yingli Zhou ; Li Wang ; Lei Han ; Jin’e Lei…
- 刊名：Current Microbiology
- 出版年：2015
- 出版时间：April 2015
- 年：2015
- 卷：70
- 期：4
- 页码：528-535
- 全文大小：1,963 KB
- 参考文献：1. Bertoloni, G, Lauro, FM, Cortella, G, Merchat, M (2000) Photosensitizing activity of hematoporphyrin on Staphylococcus aureus cells. Biochim Biophys Acta 1475: pp. 169-174 CrossRef
2. Caminos, DA, Spesia, MB, Pons, P, Duraniti, EN (2008) Mechanisms of Escherichia coli photodynamic inactivation by an amphiphilic tricationic porphyrin and 5, 10, 15, 20-tetra (4-N,N,N-trimethylammoniumphenyl) porphyrin. Photochem Photobiol Sci 7: pp. 1071-1078 CrossRef
3. Capella, M, Coelho, AM, Menezes, S (1996) Effect of glucose on photodynamic action of methylene blue in Escherichia coli cells. Photochem Photobiol 64: pp. 205-210 CrossRef
4. Cassidy, CM, Tunney, MM, McCarron, PA, Donnelly, RF (2009) Drug delivery strategies for photodynamic antimicrobial chemotherapy: from benchtop to clinical practice. J Photochem Photobiol B 95: pp. 71-80 CrossRef
5. Castano, AP, Mroz, P, Hamblin, MR (2006) Photodynamic therapy and anti-tumor immunity. Nat Rev Cancer 6: pp. 535-545 CrossRef
6. Celli, JP, Spring, BQ, Rizvi, I, Evans, CL, Samkoe, KS, Verma, S, Pogue, BW, Hasan, T (2010) Imaging and photodynamic therapy: mechanisms, monitoring, and optimization. Chem Rev 110: pp. 2795-2838 CrossRef
7. Chen, CZ, Cooper, SL (2002) Interactions between dendrimer biocides and bacterial membranes. Biomaterials 23: pp. 3359-3368 CrossRef
8. Choi, SS, Lee, HK, Chae, HS (2012) Comparison of in vitro photodynamic antimicrobial activity of protoporphyrin IX between endoscopic white light and newly developed narrowband endoscopic light against Helicobacter pylori 26695. J Photochem Photobiol B 117: pp. 55-60 CrossRef
9. Demidova, T, Hamblin, M (2005) Effects of cell-photosensitizer binding and cell density on microbial photoinactivation. Antimicrob Agents Chemother 6: pp. 2329-2335 CrossRef
10. Denis, TG, St, Dai T, Izikson, L, Astrakas, C, Anderson, RR, Hamblin, MR, Tegos, GP (2011) All you need is light: antimicrobial photoinactivation as an evolving and emerging discovery strategy against infectious disease. Virulence 2: pp. 509-520 CrossRef
11. Ferro, S, Ricchelli, F, Monti, D, Mancini, G, Jori, G (2007) Efficient photoinactivation of methicillin-resistant Staphylococcus aureus by a novel porphyrin incorporated into a poly-cationic liposome. Int J Biochem Cell B 39: pp. 1026-1034 CrossRef
12. Figueiredo, RA, Anami, LC, Mello, I, Carvalho, ED, Habitante, SM, Raldi, DP (2014) Tooth discoloration induced by endodontic phenothiazine dyes in photodynamic therapy. Photomed Laser Surg 32: pp. 458-462 CrossRef
13. Fotinos, N, Campo, MA, Popowycz, F, Gurny, R, Lange, N (2006) 5-Aminolevulinic acid derivatives in photomedicine: characteristics, application and perspectives. Photochem Photobiol 82: pp. 994-1015 CrossRef
14. Fotinos, N, Convert, M, Piffaretti, JC, Gurny, R, Lange, N (2008) Effects on Gram-negative and Gram-positive bacteria mediated by 5-aminolevulinic acid and 5-aminolevulinic acid derivatives. Animicrob Agents Chemother 52: pp. 1366-1373 CrossRef
15. Gad, F, Zahra, T, Hasan, T, Hamblin, MR (2004) Effects of growth phase and extracellular slime on photodynamic inactivation of Gram-positive pathogenic bacteria. Animicrob Agents Chemother 48: pp. 2173-2178 Life Sciences - 刊物主题：Life Sciences
Microbiology
Biotechnology - 出版者：Springer New York
- ISSN：1432-0991
文摘
Vancomycin-resistant Enterococci (VRE) is a serious concern for public health. Serious infections with VRE have very limited effective antimicrobial therapy, and alternative treatment approaches are highly desirable. One promising approach might be the photodynamic antimicrobial chemotherapy. In the present study, we investigated the photodynamic inactivation (PDI) of two VRE strains mediated by 5-aminolevulinic acid (5-ALA) and its derivative 5-ALA methyl ester (MAL). The photodynamic damages to bacteria on the level of genomic DNA, the leakage of cell components, and the changes of membrane structure were investigated. After treated with 10?mM 5-ALA and irradiated by the 633?±?10?nm LED for 60?min, 5.37 and 5.22 log10 reductions in bacterial survival were achieved for the clinical isolate of VRE and E. faecalis (ATCC 51299), respectively. After treated with 10?mM MAL and irradiated by the LED for 60?min, 5.02 and 4.91 log10 reductions in bacterial survival were observed for the two VRE strains, respectively. In addition, the photocleavage on genomic DNA and the rapid release of intracellular biopolymers were detected in PDI-treated bacteria. The intensely denatured cytoplasm and the aggregated ribosomes were also found in PDI-treated bacteria by transmission electron microscopy. Although 5-ALA and MAL-mediated PDI could induce the photocleavage on genomic DNA, the PDI of the two VRE strains might be predominantly attributed to the envelope injury, the intracellular biopolymers leakage, and the cytoplasm denature.