Eggplant (Solanum melongena L.) polyphenol oxidase multi-gene family: a phylogenetic evaluation

详细信息    查看全文

• 作者：Aravind Kumar Jukanti ; Ramakrishna Bhatt
• 关键词：Eggplant ; Polyphenol oxidase ; Phylogenetic analysis ; Multi ; gene family ; N ; glycosylation
• 刊名：3 Biotech
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：5
• 期：1
• 页码：93-99
• 全文大小：2,993 KB
• 参考文献：1. Anderson JA,Morris CF (2001) An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity. Crop Sci 41:1697-705 CrossRef
  2. Baik BK, Czuchajowska Z, Pomeranz Y (1994) Comparison of polyphenol oxidase activities in wheats and flours from Australian and US cultivars. J Cereal Sci 19:291-96 CrossRef
  3. Brendel V, Bucher P, Nourbakhsh IR, Blaisdell BE, Karlin S (1992) Methods and algorithms for statistical analysis of protein sequences. Proc Natl Acad Sci USA 89:2002-006 CrossRef
  4. Chevalier T, de Rigal D, Mbeguie AMD, Gauillard F, Richard-Forget F, Fils-Lycaon BR (1999) Molecular cloning and characterization of apricot fruit polyphenol oxidase. Plant Physiol 119:1261-270 CrossRef
  5. Constabel CP, Bergey D, Ryan CA (1995) Systemin activates synthesis of wound-inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathway. Proc Natl Acad Sci USA 92:407-11 CrossRef
  6. Dry IB, Robinson SP (1994) Molecular cloning and characterization of grape berry polyphenol oxidase. Plant Mol Biol 26:495-02 CrossRef
  7. Feillet P, Autran J-C, Icard-Verniere C (2000) Pasta brownness: an assessment. J Cereal Sci 32:215-33 CrossRef
  8. Felsenstein J (1989) PHYLIP—Phylogeny Inference Package (Version 3.2). Cladistics 5:164-66
  9. Hunt MD, Eannetta NT, Yu H, Newman SM, Steffens JC (1993) cDNA cloning and expression of potato polyphenol oxidase. Plant Mol Biol 21:59-8 CrossRef
  10. Joy RW, Sugiyama M, Fukuda H, Komamine A (1995) Cloning and characterization of polyphenol oxidase cDNAs of / Phytolacca americana. Plant Physiol 107:1083-089 CrossRef
  11. Koussevitzky S, Ne’eman E, Sommer A, Steffens JC, Harel E (1998) Purification and properties of a novel chloroplast stromal peptidase. Processing of polyphenol oxidase and other imported precursors. J Biol Chem 273:27064-7069 CrossRef
  12. Massa AN, Beecher B, Morris CF (2007) Polyphenol oxidase (PPO) in wheat and wild relatives: molecular evidence for a multigene family. Theor Appl Genet 114:1239-247 CrossRef
  13. Mayer AM, Harel E (1979) Polyphenol oxidases in plants. Phytochemistry 18:193-15 CrossRef
  14. Ono E, Hatayama M, Isono Y, Sato T, Watanabe R, Yonekura-Sakakibara K, Fukuchi-Mizutani M, Tanaka Y, Kusumi T, Nishino T, Nakayama T (2006) Localization of a flavonoid biosynthetic polyphenol oxidase in vacuoles. Plant J 45:133-43 CrossRef
  15. Pérez-Gilabert M, García Carmona F (2000) Characterization of catecholase and cresolase activities of eggplant polyphenol oxidase. J Agric Food Chem 48:695-00 CrossRef
  16. Pourcel L, Routaboul JM, Kerhoas L, Caboche M, Lepiniec L, Debeaujon I (2005) TRANSPARENT TESTA10 encodes a laccase-like enzyme involved in oxidative polymerization of flavonoids in Arabidopsis seed coat. Plant Cell 17:2966-980 CrossRef
  17. Shahar T, Hennig N, Gutfinger T, Hareven D, Lifschitz E (1992) The tomato 66.3-kD polyphenoloxidase gene: molecular identification and developmental expression. Plant Cell 4:135-47 CrossRef
  18. Shetty SM, Chandrashekar
• 刊物主题：Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-5738

文摘

Polyphenol oxidases (PPOs) in different Solanum species including eggplant have been studied. PPOs have been implicated in undesirable enzymatic browning of eggplant fruit and also in plant defense. The main objective of this study was to identify and accelerate the further functional characterization of additional eggplant PPOs that are involved in food biochemistry and defense-related functions. Eggplant PPOs identified earlier were used in “Basic local alignment search tool (BLAST)-search against expressed sequence tag and nucleotide databases. We have identified seven additional sequences which were almost complete in length. The sequences of the PPOs were aligned and their phylogenetic and evolutionary relationships established. The sequences are quite diverse, broadly falling into two major clusters; three PPOs form a separate branch/minor cluster. The thirteen sequences had conserved copper A binding sites but copper B binding sites differed considerably in two new PPO sequences (AFJ79642 and ACR61398). A third conserved ‘Histidine-rich-region has been identified at the ‘C-terminus of the eggplant PPOs. In addition, all the seven new PPOs exhibited at least one glycosylated sequon in the mature PPO sequence. Identification of additional PPO genes will further help in functional and biological characterization of these PPOs.