Metabolomic study of the bone trabecula of osteonecrosis femoral head patients based on UPLC–MS/MS

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• 作者：Weiwen Zhu ; Tingmei Chen ; Shijia Ding ; Gang Yang ; Zhongwei Xu ; Kaimin Xu…
• 关键词：Metabolomics ; UPLC–MS/MS ; Osteonecrosis of the femoral head ; Biomarkers
• 刊名：Metabolomics
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：12
• 期：3
• 全文大小：1,679 KB
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• 作者单位：Weiwen Zhu (1)
  Tingmei Chen (2)
  Shijia Ding (2)
  Gang Yang (1)
  Zhongwei Xu (1)
  Kaimin Xu (1)
  Shengyu Zhang (1)
  Tao Ma (1)
  Jian Zhang (1)
  
  1. Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China
  2. Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China
  
• 刊物主题：Biochemistry, general; Molecular Medicine; Cell Biology; Developmental Biology; Biomedicine general;
• 出版者：Springer US
• ISSN：1573-3890

文摘

The severity and/or progression of osteonecrosis of the femoral head (ONFH) are commonly assessed by radiography, nuclear magnetic resonance image which aren’t invariably correlated to severity of disease and may be disturbed by other factors. Consequently, exploring the novel biochemical signatures of ONFH may be beneficial for diagnosing and understanding this disease. In this work, a bone trabecula metabolomics was undertaken to determine the expression pattern of low molecular mass metabolites in patients of femoral head necrosis based on the ultra-performance liquid chromatography/time-of-flight tandem mass spectrometry (UPLC/TOF MS/MS). Histological study showed that necrotic bone was characterized by necrosis, fibrosis and lacuna, but adjacent “normal” bone was pathologically normal. Principal component analysis in combination with orthogonal partial least-squares discrimination analysis was used to find out changed metabolites. MS/MS was used to speculate the corresponding molecule. Both osteonecrotic bone trabecula (ONBT) and adjacent “normal” bone trabecula (ANBT) showed higher levels of amino acids, such as proline, arginine, glutamine, dipeptides and lower levels of antioxidants. Most disrupted lipids, such as fatty acid esters, glycerophospholipids, sphingolipids, were found in osteonecrotic zone. The area under the receiver operating characteristic curve of combinational biomarkers (d-arginine, l-proline, l-carnitine, inosine) in ONBT and ANBT was 0.996 and 0.950, respectively. Our findings might provide a significant insight to understand the metabolic mechanism and diagnosis biomarkers of ONFH in the future. Keywords Metabolomics UPLC–MS/MS Osteonecrosis of the femoral head Biomarkers