Hexameric molecular motors: P4 packaging ATPase unravels the mechanism

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• 作者：D. E. Kainov ; R. Tuma and E. J. Mancini
• 关键词：Genome packaging ; RecA ; like motor ; hexameric helicase ; dsRNA bacteriophage ; virus assembly
• 刊名：Cellular and Molecular Life Sciences (CMLS)
• 出版年：2006
• 出版时间：May, 2006
• 年：2006
• 卷：63
• 期：10
• 页码：1095-1105
• 全文大小：602 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Life Sciences
  Biochemistry
  
• 出版者：Birkh盲user Basel
• ISSN：1420-9071

文摘

Genome packaging into an empty capsid is an essential step in the assembly of many complex viruses. In double-stranded RNA (dsRNA) bacteriophages of the Cystoviridae family this step is performed by a hexameric helicase P4 which is one of the simplest packaging motors found in nature. Biochemical and structural studies of P4 proteins have led to a surprising finding that these proteins bear mechanistic and structural similarities to a variety of the pervasive RecA/F1-ATPase-like motors that are involved in diverse biological functions. This review describes the role of P4 proteins in assembly, transcription and replication of dsRNA bacteriophages as it has emerged over the past decade while focusing on the most recent structural studies. The P4 mechanism is compared with the models proposed for the related hexameric motors.