Electrospun poly(d/l-lactide-co-l-lactide) hybrid matrix: a novel s

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• 作者：Petra J. Kluger ; Ralf Wyrwa ; J¨¹rgen Weisser ; Julia Maierle ; Miriam Votteler ; Claudia Rode ; Matthias Schnabelrauch ; Heike Walles and Katja Schenke-Layland
• 刊名：Journal of Materials Science Materials in Medicine
• 出版年：2010
• 出版时间：September 2010
• 年：2010
• 卷：21
• 期：9
• 页码：2665-2671
• 全文大小：503.2 KB

文摘

Electrospinning is a long-known polymer processing technique that has received more interest and attention in recent years due to its versatility and potential use in the field of biomedical research. The fabrication of three-dimensional (3D) electrospun matrices for drug delivery and tissue engineering is of particular interest. In the present study, we identified optimal conditions to generate novel electrospun polymeric scaffolds composed of poly-d/l-lactide and poly-l-lactide in the ratio 50:50. Scanning electron microscopic analyses revealed that the generated poly(d/l-lactide-co-l-lactide) electrospun hybrid microfibers possessed a unique porous high surface area mimicking native extracellular matrix (ECM). To assess cytocompatibility, we isolated dermal fibroblasts from human skin biopsies. After 5 days of in vitro culture, the fibroblasts adhered, migrated and proliferated on the newly created 3D scaffolds. Our data demonstrate the applicability of electrospun poly(d/l-lactide-co-l-lactide) scaffolds to serve as substrates for regenerative medicine applications with special focus on skin tissue engineering.