Immunization with Wuchereria bancrofti Glutathione-S-transferase Elicits a Mixed Th1/Th2 Type of Protective Immune Response Against Filarial Infection in Mastomys

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• 作者：Dhananjay Andure ; Kiran Pote ; Vishal Khatri…
• 关键词：Wuchereria bancrofti ; Brugia malayi ; Glutathione ; S ; transferases ; Vaccine ; Nematodes
• 刊名：Indian Journal of Clinical Biochemistry
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：31
• 期：4
• 页码：423-430
• 全文大小：750 KB
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• 作者单位：Dhananjay Andure (1)
  Kiran Pote (2)
  Vishal Khatri (2)
  Nitin Amdare (2)
  Ramchandra Padalkar (1)
  Maryada Venkata Rami Reddy (2)
  
  1. Padmashree Dr. Vithalrao Vikhe Patil Foundation’s Medical College and Hospital, Ahmednagar, Maharashtra, 414 111, India
  2. Department of Biochemistry and JB Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Maharashtra, 442 102, India
  
• 刊物主题：Biochemistry, general; Microbiology; Chemistry/Food Science, general; Pathology;
• 出版者：Springer India
• ISSN：0974-0422
• 卷排序：31

文摘

Lymphatic filariasis is a mosquito borne parasitic infection and can severely affect the normal working ability of an individual. Currently there is no vaccine available to prevent this infection and the development of a potential vaccine could effectively support the on-going mass drug administration program by World Health Organization (WHO). Filarial parasites have complex mechanisms to modulate the host immune responses against them. The glutathione-S-transferases (GST) are the important enzymes effectively involved to counteract the oxidative free radicals produced by the host. In the present study, we have shown that the mastomys which are fully permissible rodents for Brugia malayi when immunized with Wuchereria bancrofti recombinant GST (rWbGST) could induce 65.5 % in situ cytotoxicity against B. malayi infective (L₃) larvae. There was a balanced Th1/Th2 immune response in the vaccinated animals, characterized by higher levels of WbGST-specific IgG1 and IgG2a antibodies and pronounced IFN-γ, IL-10 and IL-4 cytokines production by the spleen cells. Keywords Wuchereria bancrofti Brugia malayi Glutathione-S-transferases Vaccine Nematodes