Performance of trichlorfon degradation by a novel Bacillus tequilensis strain PA F-3 and its proposed biodegradation pathway

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• 作者：Jiang Tian ; Chenlei Yu ; Yingwen Xue ; Ruixue Zhao ; Jing Wang ; Lanzhou Chen
• 关键词：Trichlorfon (TCF) ; TCF degrading bacterium ; Bacillus tequilensis ; Biodegradation products ; Biodegradation pathway
• 刊名：Biodegradation
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：27
• 期：4-6
• 页码：265-276
• 全文大小：1,037 KB
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Soil Science and Conservation
  Geochemistry
  Terrestrial Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Waste Management and Waste Technology
  
• 出版者：Springer Netherlands
• ISSN：1572-9729
• 卷排序：27

文摘

The novel trichlorfon (TCF)-degrading bacterium PA F-3, identified as Bacillus tequilensis, was isolated from pesticide-polluted soils by using an effective screening and domesticating procedure. The TCF biodegradation pathways of PA F-3 were also systematically elucidated. As revealed by high-performance liquid chromatography, the TCF residues in the mineral salt medium demonstrated that PA F-3 can utilize TCF as its sole carbon source and reach the highest degradation of 71.1 % at an initial TCF concentration of 200 mg/L within 5 days. The TCF degradation conditions were optimized using response surface methodology as follows: temperature, 28 °C; inoculum amount, 4 %; and initial TCF concentration, 125 mg/L. Biodegradation treatments supplemented with exogenous carbon sources and yeast extract markedly increased the microbial dry weights and TCF-degrading performance of PA F-3, respectively. Meanwhile, five metabolic products of TCF were identified through gas chromatography/mass spectrometry, and a biodegradation pathway was proposed. Results indicated that deoxidation and dehydration (including the cleavage of the P–C phosphonate bond and the C–O bond) were the preferred metabolic reactions of TCF in this TCF-degrading bacterium.