Effects of different fluences of low-level laser therapy in an experimental model of spinal cord injury in rats
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- 作者：Suellen Veronez ; Lívia Assis ; Paula Del Campo…
- 关键词：Low ; level laser therapy ; Spinal cord injury ; Neuronal plasticity ; Wistar
- 刊名：Lasers in Medical Science
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：32
- 期：2
- 页码：343-349
- 全文大小：
- 刊物类别：Medicine
- 刊物主题：Medicine/Public Health, general; Dentistry; Optics, Lasers, Photonics, Optical Devices; Quantum Optics;
- 出版者：Springer London
- ISSN：1435-604X
- 卷排序：32
文摘
The aim of this study was to evaluate the in vivo response of different fluences of low-level laser therapy (LLLT) on the area of the injury, inflammatory markers, and functional recovery using an experimental model of traumatic spinal cord injury (SCI). Thirty two rats were randomly divided into four experimental groups: control group (CG), laser-treated group 500 J/cm2 (L-500), laser-treated group 750 J/cm2 (L-750), and laser-treated group 1000 J/cm2 (L-1000). SCI was performed by an impactor equipment (between the ninth and tenth thoracic vertebrae), with a pressure of 150 kdyn. Afterwards, the injured region was irradiated daily for seven consecutive sessions, using an 808-nm laser, at the respective fluence of each experimental groups. Motor function and tactile sensitivity were performed on days 1 and 7 post-surgery. Animals were euthanized on the eighth day after injury, and the samples were retrieved for histological and immunohistochemistry analyses. Functional evaluation and tactile sensitivity were improved after LLLT, at the higher fluence. Additionally, LLLT, at 750 and 1000 J/cm2, reduces the lesion volume and modulates the inflammatory process with decrease of CD-68 protein expression. These results suggest that LLLT at higher doses was effective in promoting functional recovery and modulating inflammatory process in the spinal cord of rats after SCI.