Licochalcone A as a potent antitumor agent suppresses growth of human oral cancer SCC-25 cells in vitro via caspase-3 dependent pathways

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• 作者：Guang Zeng (1)
  Huan Shen (2)
  Yongjin Yang (2)
  Xingwei Cai (2)
  Wenxing Xun (1)
  
• 关键词：Oral squamous cell carcinoma ; Licochalcone A ; Apoptosis ; Caspase cascade ; DNA fragmentation ; PARP cleavage
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：35
• 期：7
• 页码：6549-6555
• 全文大小：3,049 KB
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• 作者单位：Guang Zeng (1)
  Huan Shen (2)
  Yongjin Yang (2)
  Xingwei Cai (2)
  Wenxing Xun (1)
  
  1. Department of Plastic and Burn Surgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, 710038, People’s Republic of China
  2. Department of Stomatology, The General Hospital of the Second Artillery Corps of Chinese PLA, Beijing, 100088, People’s Republic of China
  
• ISSN：1423-0380

文摘

The majority of anticancer drugs are of natural origin. However, it is unknown whether licochalcone A is cytotoxic towards oral squamous cell carcinoma (OSCC) cells. The goal of this study was to investigate the cytotoxic effects of licochalcone A on the human OSCC SCC-25 cells and to identify the underlying molecular mechanism. Exposure of SCC-25 cells to licochalcone A dose- and time-dependently decreased cell viability by arresting cell cycle at the S and G2/M phase as well as inducing apoptosis. Furthermore, the proapoptotic activity of licochalcone A was revealed by DNA fragmentation. Concomitantly, we observed activation of the effector caspases-3, induced by activation of the initiator caspases -8 and -9, which subsequent trigger both death receptor pathway and the mitochondrial apoptotic pathway in licochalcone A-mediated SCC-25 cell apoptosis. Besides, treatment with 50?μg/mL of licochalcone A for 36?h led to the cleavage of PARP, an indicator of apoptosis induction. Therefore licochalcone A may be a good candidate for development as a possible chemopreventive agent against OSCC.