Pseudoxanthomonas gei sp. nov., a novel endophytic bacterium isolated from the stem of Geum aleppicum

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• 作者：Lei Zhang (1)
  Linfang Wei (1)
  Lingfang Zhu (1)
  Changfu Li (1)
  Yao Wang (1)
  Xihui Shen (1)
  
• 关键词：Pseudoxanthomonas gei sp. nov. ; Endophytic bacterium ; Stem of Geum aleppicum ; Polyphasic approach
• 刊名：Antonie van Leeuwenhoek
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：105
• 期：4
• 页码：653-661
• 全文大小：287 KB
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• 作者单位：Lei Zhang (1)
  Linfang Wei (1)
  Lingfang Zhu (1)
  Changfu Li (1)
  Yao Wang (1)
  Xihui Shen (1)
  
  1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China
  
• ISSN：1572-9699

文摘

A Gram stain-negative, strictly aerobic, rod-shaped, non-motile and deep-yellow-coloured bacterial strain, designated ZFJR-3T, was isolated from the stem of Geum aleppicum Jacq. collected from Taibai Mountain in Shaanxi Province, north-west China, and characterized by using a polyphasic approach. The novel isolate grew optimally at 25-8?°C and in the absence of NaCl. Flexirubin-type pigments were produced. The predominant respiratory quinone was ubiquinone-8 (Q-8) and the major cellular fatty acids were iso-C15:0 (29.2?%), iso-C16:0 (18.5?%), summed feature 9 (comprising iso-C17:1 ω9c and/or C16:0 10-methyl; 8.8?%), C16:1 ω7c alcohol (8.8?%), iso-C11:0 3-OH (6.9?%) and iso-C11:0 (6.8?%). The DNA G+C content was 66.1?mol?%. The only polyamine was spermidine and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZFJR-3T belongs to the genus Pseudoxanthomonas and was most closely related to Pseudoxanthomonas yeongjuensis KCTC 22757T (16S rRNA gene sequence similarity, 99.0?%) and Pseudoxanthomonas sacheonensis KCTC 22080T (98.0?%). The levels of 16S rRNA gene sequence similarity with respect to other Pseudoxanthomonas species with validly published names were less than 96.5?%. DNA–DNA relatedness values for strain ZFJR-3T with respect to its closely related neighbours P. yeongjuensis KCTC 22757T and P. sacheonensis KCTC 22080T were 48.7 and 36.3?%, respectively. Based on the phenotypic, phylogenetic and genotypic data, strain ZFJR-3T is considered to represent a novel species of the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas gei sp. nov. is proposed. The type strain is ZFJR-3T (=CCTCC AB 2013020T =KCTC 32298T).