Transcriptome profiling of indole-3-butyric acid-induced adventitious root formation in softwood cuttings of the Catalpa bungei variety ‘YU-1’ at different developmental stages

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• 作者：Peng Wang ; Lingling Ma ; Ya Li ; Shu’an Wang ; Linfang Li ; Rutong Yang…
• 关键词：RNA ; Seq ; Softwood cutting ; Plant hormone ; qRT ; PCR ; Gene expression
• 刊名：Genes & Genomics
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：38
• 期：2
• 页码：145-162
• 全文大小：2,488 KB
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• 作者单位：Peng Wang (1)
  Lingling Ma (1)
  Ya Li (1)
  Shu’an Wang (1)
  Linfang Li (1)
  Rutong Yang (1)
  Yuzhu Ma (1)
  Qing Wang (1)
  
  1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China
  
• 刊物主题：Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Human Genetics;
• 出版者：Springer Netherlands
• ISSN：2092-9293

文摘

Catalpa bungei is a deciduous tree native to China. It is characterized as fast growing, being highly adaptable, and having excellent wood qualities. To better understand potential mechanisms involved in adventitious root (AR) formation, we performed transcriptome analysis of softwood cuttings of C. bungei ‘Yu-1’ at three stages of AR formation using the Illumina sequencing method. Following de novo assembly, 62,955 unigenes were obtained, 31,646 (50.26 %) of which were annotated. A total of 11,100 differentially expressed genes (DEGs), including 10,200 unique and 900 common, were identified in four comparisons. Based on the all GO enrichment networks, 46 common and 7 unique GO categories were identified. Cytoskeleton was only significantly enriched in the activation period, while DNA metabolic process was only significantly enriched in the callus formation. Functional annotation analysis revealed that many of these genes were involved in phenylpropanoid biosynthesis, glycolysis, and plant hormone metabolism, suggesting potential contributions to AR formation. Interestingly, the number of DEGs involved in glycolysis decreased while the number of DEGs involved in phenylpropanoid biosynthesis increased following the AR formative process. Overall, our comprehensive transcriptional overview will prove useful, not only in the understanding of molecular networks that regulate AR formation in C. bungei, but also for exploring genes that may improve rooting rates of other trees. Keywords RNA-Seq Softwood cutting Plant hormone qRT-PCR Gene expression