Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases
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- 作者：Péter Lajos Sóti ; Diana Weiser ; Tamás Vigh…
- 关键词：Biocatalysis ; Electrospinning ; Immobilization ; Lipase ; Kinetic resolution
- 刊名：Bioprocess and Biosystems Engineering
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：39
- 期：3
- 页码：449-459
- 全文大小：1,214 KB
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Diana Weiser (1)
Tamás Vigh (1)
Zsombor Kristóf Nagy (1)
László Poppe (1) (2)
György Marosi (1)
1. Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Budafoki út 8., 1111, Budapest, Hungary
2. SynBiocat Ltd., Lázár deák u. 4/1, 1173, Budapest, Hungary - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Industrial Chemistry and Chemical Engineering
Industrial and Production Engineering
Waste Management and Waste Technology
Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
Food Science - 出版者：Springer Berlin / Heidelberg
- ISSN：1615-7605
文摘
Electrospinning was applied to create easy-to-handle and high-surface-area membranes from continuous nanofibers of polyvinyl alcohol (PVA) or polylactic acid (PLA). Lipase PS from Burkholderia cepacia and Lipase B from Candida antarctica (CaLB) could be immobilized effectively by adsorption onto the fibrous material as well as by entrapment within the electrospun nanofibers. The biocatalytic performance of the resulting membrane biocatalysts was evaluated in the kinetic resolution of racemic 1-phenylethanol (rac-1) and 1-phenylethyl acetate (rac-2). Fine dispersion of the enzymes in the polymer matrix and large surface area of the nanofibers resulted in an enormous increase in the activity of the membrane biocatalyst compared to the non-immobilized crude powder forms of the lipases. PLA as fiber-forming polymer for lipase immobilization performed better than PVA in all aspects. Recycling studies with the various forms of electrospun membrane biocatalysts in ten cycles of the acylation and hydrolysis reactions indicated excellent stability of this forms of immobilized lipases. PLA-entrapped lipases could preserve lipase activity and enantiomer selectivity much better than the PVA-entrapped forms. The electrospun membrane forms of CaLB showed high mechanical stability in the repeated acylations and hydrolyses than commercial forms of CaLB immobilized on polyacrylamide beads (Novozyme 435 and IMMCALB-T2-150).