Mass spectrometric studies on the interaction of cisplatin and insulin

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• 作者：Jing Li ; Lei Yue ; Yaqin Liu ; Xinchi Yin ; Qi Yin ; Yuanjiang Pan ; Lirong Yang
• 关键词：Insulin from porcine pancreas ; Cisplatin ; Insulin–cisplatin adduct ; Mass spectrometry ; Binding sites
• 刊名：Amino Acids
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：48
• 期：4
• 页码：1033-1043
• 全文大小：660 KB
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• 作者单位：Jing Li (1) (2)
  Lei Yue (2)
  Yaqin Liu (2)
  Xinchi Yin (2)
  Qi Yin (2)
  Yuanjiang Pan (2)
  Lirong Yang (1)
  
  1. Department of Chemical and Biological Engineering, Institute of Biological Engineering, Zhejiang University, Hangzhou, 310027, Zhejiang, China
  2. Department of Chemistry, Zhejiang University, Hangzhou, 310027, Zhejiang, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Analytical Chemistry
  Biochemical Engineering
  Life Sciences
  Proteomics
  Neurobiology
  
• 出版者：Springer Wien
• ISSN：1438-2199

文摘

The interaction of antitumor drug, cisplatin (cis-[PtCl₂(NH₃)₂], CDDP) with insulin from porcine pancreas has been investigated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and high resolution hybrid ion trap/time-of-flight mass spectrometry (MALIDI-TOF/TOF–MS and ESI-IT/TOF MS). The MALDI-TOF/TOF–MS results demonstrated that the presence of cisplatin complex resulted in the reduction of the disulfide bond in porcine pancreas after the incubations of the two substances were performed in vitro. It indicated that the presence of cisplatin would destroy the native configuration of insulin, which may lead to the inactivation of insulin. High resolution mass values and the characteristic isotopic pattern of the platinated insulin ions allowed the analysis of platinated mono-, di- and triadducts of cisplatin and insulin in the incubations under different conditions. The laser-induced dissociation of the monoadduct obtained in MALDI source was carried out and one platinum was found to bind to insulin B chain was determined. The platinum binding sites were further identified to be the N terminus (B chain), cysteine 7 (B chain) and cysteine 19 (B chain) residues by electrospray ionization tandem mass spectrometry. The identification of the interaction between insulin and cisplatin broadens the horizon of the knowledge in the interaction of the proteins and metallodrugs.