Molecular responses of cells to 2-mercapto-1-methylimidazole gold nanoparticles (AuNPs)-mmi: investigations of histone methylation changes

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• 作者：Arianna Polverino (1)
  Angela Longo (2)
  Aldo Donizetti (3)
  Denise Drongitis (3)
  Maria Frucci (1) (5)
  Loredana Schiavo (2)
  Gianfranco Carotenuto (2)
  Luigi Nicolais (4)
  Marina Piscopo (3)
  Emilia Vitale (1) (6)
  Laura Fucci (3)
  
  1. Institute of Cybernetics ; National Research Council (CNR) of Pozzuoli ; Naples ; Italy
  2. Institute for Polymers ; Composites and Biomaterials ; National Research Council (CNR) ; Naples ; Italy
  3. Department of Biology ; University of Naples Federico II ; Naples ; Italy
  5. Institute for High-Performance Computing and Networking ; National Research Council (CNR) ; Naples ; Italy
  4. Department of Chemical ; Materials and Industrial Engineering DICMAPI ; University of Naples Federico II ; Naples ; Italy
  6. Institute of Protein Biochemistry (IBP) ; National Research Council (CNR) ; Via Pietro Castellino n. 111 ; 80131 ; Naples ; Italy
  
• 关键词：Gold nanoparticles (AuNPs) ; (AuNPs) ; mmi ; Histone dimethylation ; Chromatin
• 刊名：Journal of Nanoparticle Research
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：16
• 期：7
• 全文大小：1,263 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nanotechnology
  Inorganic Chemistry
  Characterization and Evaluation Materials
  Physical Chemistry
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer Netherlands
• ISSN：1572-896X

文摘

While nanomedicine has an enormous potential to improve the precision of specific therapy, the ability to efficiently deliver these materials to regions of disease in vivo remains limited. In this study, we describe analyses of (AuNPs)-mmi cellular intake via fluorescence microscopy and its effects on H3K4 and H3K9 histone dimethylation. Specifically, we studied the level of H3K4 dimethylation in serving the role of an epigenetic marker of euchromatin, and of H3K9 dimethylation as a marker of transcriptional repression in four different cell lines. We analyzed histone di-methyl-H3K4 and di-methyl-H3K9 using either variable concentrations of nanoparticles or variable time points after cellular uptake. The observed methylation effects decreased consistently with decreasing (AuNPs)-mmi concentrations. Fluorescent microscopy and a binarization algorithm based on a thresholding process with RGB input images demonstrated the continued presence of (AuNPs)-mmi in cells at the lowest concentration used. Furthermore, our results show that the treated cell line used is able to rescue the untreated cell phenotype.