Copro prevalence and estimated risk of Entamoeba histolytica in Diarrheic patients at Beni-Suef, Egypt

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• 作者：Shimaa S. Ibrahim ; Olfat M. El-Matarawy…
• 关键词：E. histolytica ; E. histolytica/E. dispar ; Entamoeba complex ; Microscopy ; Immunocytochromatograghy ; ELISA
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：31
• 期：2
• 页码：385-390
• 全文大小：197 KB
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• 作者单位：Shimaa S. Ibrahim (1)
  Olfat M. El-Matarawy (2)
  Marwa A. Ghieth (1)
  Enas Y. Abu Sarea (1)
  Ayman A. El-Badry (2)
  
  1. Department of Medical Parasitology, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt
  2. Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, El-Manial, Cairo, Egypt
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

Amoebiasis diagnosis is usually based on microscopy that cannot differentiate pathogenic E. histolytica from morphologically identical non-pathogenic species. 194 fecal samples were collected from diarrheic &/or dysenteric patients and examined for Entamoeba complex microscopically, E. histolytica/E. dispar coproantigen using ICT and E. histolytica coproantigen using Tech lab E. histolytica II ELISA test. Entamoeba complex trophozoites/cysts, E. histolytica/E. dispar coproantigen and E. histolytica coproantigen were detected in 22.2, 14.4 and 3.6?% of samples, respectively. Microscopy and ICT method had limited sensitivity with poor PPV (9.3 and 7.1?%, respectively) and both slightly agree with ELISA test. The prevalence of E. histolytica was low (3.6?%) in studied individuals and was 14 times lower than non-pathogenic amoebae. E. histolytica detection studied individuals was positively associated with mucoid and bloody stool, which makes them disease predictors. E. histolytica fecal ELISA assay for E. histolytica detection surpassed microscopy and E. histolytica/E. dispar ICT assay. This has highlighted the need for practical non-microscopic detection methods that can differentiate between amoeba infections to avoid unnecessary and possibly harmful therapies and to determine the true prevalence and epidemiology of E. histolytica.