New approach to the mitotype classification in black honeybee Apis mellifera mellifera and Iberian honeybee Apis mellifera iberiensis

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• 作者：R. A. Ilyasov ; A. V. Poskryakov ; A. V. Petukhov…
• 关键词：black honeybee ; Apis melifera melifera ; evolutionary lineage M ; mtDNA COI–COII intergenic region ; mitotypic classification of honeybee ; single nucleotide polymorphism (SNP)
• 刊名：Russian Journal of Genetics
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：52
• 期：3
• 页码：281-291
• 全文大小：1,067 KB
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• 作者单位：R. A. Ilyasov (1)
  A. V. Poskryakov (1)
  A. V. Petukhov (2)
  A. G. Nikolenko (1)
  
  1. Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Ufa, Republic of Bashkortostan, 450054, Russia
  2. Department of Zoology, Perm State Humanitarian Pedagogical University, Perm, 614990, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Animal Genetics and Genomics
  Microbial Genetics and Genomics
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3369

文摘

The black honeybee Apis mellifera mellifera L. is today the only subspecies of honeybee which is suitable for commercial breeding in the climatic conditions of Northern Europe with long cold winters. The main problem of the black honeybee in Russia and European countries is the preservation of the indigenous gene pool purity, which is lost as a result of hybridization with subspecies, A. m. caucasica, A. m. carnica, A. m. carpatica, and A. m. armeniaca, introduced from southern regions. Genetic identification of the subspecies will reduce the extent of hybridization and provide the gene pool conservation of the black honeybee. Modern classification of the honeybee mitotypes is mainly based on the combined use of the DraI restriction endonuclease recognition site polymorphism and sequence polymorphism of the mtDNA COI–COII region. We performed a comparative analysis of the mtDNA COI–COII region sequence polymorphism in the honeybees of the evolutionary lineage M from Ural and West European populations of black honeybee A. m. mellifera and Spanish bee A. m. iberiensis. A new approach to the classification of the honeybee M mitotypes was suggested. Using this approach and on the basis of the seven most informative SNPs of the mtDNA COI–COII region, eight honeybee mitotype groups were identified. In addition, it is suggested that this approach will simplify the previously proposed complicated mitotype classification and will make it possible to assess the level of the mitotype diversity and to identify the mitotypes that are the most valuable for the honeybee breeding and rearing.