Promoter hypermethylation of the cysteine protease RECK may cause metastasis of osteosarcoma

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• 作者：Leisheng Wang ; Junbo Ge ; Tian Ma ; Yanpin Zheng ; Shiqiao Lv ; Yu Li…
• 关键词：RECK ; Promoter hypermethylation ; Metastasis ; Bone tumors ; Osteosarcoma
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：36
• 期：12
• 页码：9511-9516
• 全文大小：580 KB
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• 作者单位：Leisheng Wang (1) (2)
  Junbo Ge (2)
  Tian Ma (3)
  Yanpin Zheng (1)
  Shiqiao Lv (2)
  Yu Li (2)
  Shaoxian Liu (2)
  
  1. Department of Orthopedics, Qihu Hospital, Shandong University, Jinan, 250000, China
  2. Department of Orthopedics, Yantaishan Hospital, Yantai, 264000, China
  3. Department of Orthopedics, The People’s Hospital of Qixia, No. 259, Minsheng Road, Qixia, 265300, China
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

The present study examined the role of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) promoter hypermethylation as a causative factor in metastasis of osteosarcoma. Using human pathological samples, it is demonstrated that RECK, a cysteine protease that reversibly regulates expression of matrix metalloproteases like matrix metallopeptidase 9 (MMP9), is transcriptionally inhibited in osteosarcoma, especially metastatic variants. This result comes from its promoter hypermethylation, as evaluated in the present study by methylation-specific PCR reaction. The expression of RECK was also significantly diminished in the metastatic variants of osteosarcoma. This downregulation of RECK in advanced grades of osteosarcoma and metastatic grades was also associated with the increased expression of invadosome-specific markers like MMP9, phospho-FAK, and integrins, suggesting the complex contributions of RECK in the prevention of metastasis and its downregulation as a causative factor in osteosarcoma metastasis. Keywords RECK Promoter hypermethylation Metastasis Bone tumors Osteosarcoma