Expression of mediators of purinergic signaling in human liver cell lines

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• 作者：Jessica R. Goree (1) (2)
  Elise G. Lavoie (1) (2)
  Michel Fausther (1) (2)
  Jonathan A. Dranoff (1) (2)
  
• 关键词：Liver cell line ; Purinergic signaling ; Hepatic stellate cell ; Hepatocyte ; Cholangiocyte
• 刊名：Purinergic Signalling
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：10
• 期：4
• 页码：631-638
• 全文大小：692 KB
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• 作者单位：Jessica R. Goree (1) (2)
  Elise G. Lavoie (1) (2)
  Michel Fausther (1) (2)
  Jonathan A. Dranoff (1) (2)
  
  1. Division of Gastroenterology and Hepatology, University of Arkansas for Medical Sciences, Little Rock, AR, 72205, USA
  2. Research Service, Central Arkansas Veterans Administration Health Center, Little Rock, AR, 72205, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Pharmacology and Toxicology
  Human Physiology
  Neurosciences
  Cancer Research
  
• 出版者：Springer Netherlands
• ISSN：1573-9546

文摘

Purinergic signaling regulates a diverse and biologically relevant group of processes in the liver. However, progress of research into functions regulated by purinergic signals in the liver has been hampered by the complexity of systems probed. Specifically, there are multiple liver cell subpopulations relevant to hepatic functions, and many of these have been effectively modeled in human cell lines. Furthermore, there are more than 20 genes relevant to purinergic signaling, each of which has distinct functions. Hence, we felt the need to categorize genes relevant to purinergic signaling in the best characterized human cell line models of liver cell subpopulations. Therefore, we investigated the expression of adenosine receptor, P2X receptor, P2Y receptor, and ecto-nucleotidase genes via RT-PCR in the following cell lines: LX-2, hTERT, FH11, HepG2, Huh7, H69, and MzChA-1. We believe that our findings will provide an excellent resource to investigators seeking to define functions of purinergic signals in liver physiology and liver disease pathogenesis.