Lipid recovery from a vegetable oil emulsion using microbial enrichment cultures

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• 作者：Jelmer Tamis (1)
  Dimitry Y Sorokin (1) (2)
  Yang Jiang (1)
  Mark C M van Loosdrecht (1)
  Robbert Kleerebezem (1)
  
  1. Department of Biotechnology ; Delft University of Technology ; Julianalaan 67 ; 2628 BC ; Delft ; The Netherlands
  2. Winogradsky Institute of Microbiology ; RAS ; Leninskii avenue ; 14 ; Leninskii avenue ; 32邪 ; Moscow ; 119991 ; Russia
  
• 关键词：Vegetable oil ; Storage compounds ; Microbial enrichment culture ; Feast ; famine ; Resource recovery
• 刊名：Biotechnology for Biofuels
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：8
• 期：1
• 全文大小：2,471 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Plant Breeding/Biotechnology
  Renewable and Green Energy
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1754-6834

文摘

Background Many waste streams have a relatively high vegetable oil content, which is a potential resource that should be recovered. Microbial storage compound production for the recovery of lipids from lipid-water emulsions with open (unsterilized) microbial cultures was investigated in a sequencing batch reactor using a diluted vegetable oil emulsion as model substrate. Results After feeding, triacylglycerides (TAG) were accumulated intracellular by the microbial enrichment culture and subsequently used for growth in the remainder of the sequencing batch cycle. Roughly 50% of the added TAG could be recovered as intracellular lipids in this culture. The maximum lipid storage capacity of the enrichment culture was 54% on volatile suspended solids (VSS) mass basis in a separate fed-batch accumulation experiment. The microbial community was dominated by a lipolytic fungus, Trichosporon gracile, that was responsible for intracellular lipid accumulation but also a significant fraction of lipolytic and long chain fatty-acid-utilizing bacteria was present. Conclusion Herewith, we demonstrate an effective strategy for enrichment of a microbial community that can accumulate significant amounts of lipids from wastewaters without the need for sterilization of substrates or equipment. Further optimization of this process will make recovery of lipids from wastewater possible.