Variation in the Presence of Anti-Batrachochytrium dendrobatidis Bacteria of Amphibians Across Life Stages and Elevations in Ecuador

详细信息    查看全文

• 作者：J. C. Bresciano ; C. A. Salvador ; C. Paz-y-Mi?o ; A. M. Parody-Merino ; J. Bosch…
• 关键词：Andes ; antifungal bacteria ; biogeography ; chytridiomycosis ; life ; history ; microbiota
• 刊名：EcoHealth
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：12
• 期：2
• 页码：310-319
• 全文大小：727 KB
• 参考文献：Aranda S, Montes-Borrego M, Landa BB (2011) Purple-pigmented violacein-producing Duganella spp. Inhabit the rhizosphere of wild and cultivated olives in southern Spain. Microbial Ecology 62(2):446-59.CrossRef PubMed
  Becker MH, Brucker RM, Schwantes CR, Harris RN, Minbiole KPC (2009) The bacterially produced metabolite violacein is associated with survival of amphibians infected with a lethal fungus. Applied Environmental Microbiology 75:6635-638.PubMed Central CrossRef PubMed
  Becker MH, Harris RN (2010) Cutaneous Bacteria of the Redback Salamander Prevent Morbidity Associated with a Lethal Disease. PLoS One 5:e10957.PubMed Central CrossRef PubMed
  Becker MH, Harris RN, Minbiole KP, Schwantes CR, Rollins-Smith LA, Reinert LK, et al. (2011) Towards a better understanding of the use of probiotics for preventing chytridiomycosis in Panamanian golden frogs. Ecohealth 8:501-06.CrossRef PubMed
  Benson D, Boguski M, Lipman D, Ostell J, Ouellette B, Rapp B, Wheeler D (1999) Genbank. Nucleic Acids Research 27:12-.PubMed Central CrossRef PubMed
  Berger L, Speare R, Daszak P, Green DE, Cunninham A, Goggin CL, et al. (1998) Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proceedings of the National Academy of Sciences USA 95:9031-036.CrossRef
  Blaustein AR, Romansic JR, Scheessele EA, Han BA, Pessier AP, Longcore JE (2005) Interspecific variation in susceptibility of frog tadpoles to the pathogenic fungus, Batrachochytrium dendrobatidis. Conserv Biology 19:1460-468.CrossRef
  Bletz M, Loudon A, Becker M, Bell S, Woodhams DC, Minbiole K, Harris R (2013) Mitigating amphibian chytridiomycosis with bioaugmentation: characteristics of effective probiotics and strategies for their selection and use. Ecology letters. doi:10.-111/?ele.-2099 .PubMed
  Bosch J, Martínez-Solano I, García-París?M (2001) Evidence of a chytrid fungus infection involved in the decline of the common midwife toad (Alytes obstretricans) in protected areas of central Spain. Biological Conservation 97:331-37.CrossRef
  Bosch J, Carrascal LM, Durán L, Walker S, Fisher MC (2007) Climate change and outbreaks of amphibian chytridiomycosis in a montane area of Central Spain; is there a link? Proceedings of the Royal Society B: Biological Sciences 274:253-60CrossRef
  Boyle DG, Boyle DB, Olsen V, Morgan, JAT, Hyatt AD (2004) Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using the realtime Taqman PCR assay. Diseases of Aquatic Organisms 60:141-48.CrossRef PubMed
  Brucker RM, Baylor CM, Walters RL, Lauer A, Harris RN, Minbiole KPC (2008a) The identification of 2,4-diacetylphloroglucinol as an antifungal metabolite produced by cutaneous bacteria of the salamander?Plethodon cinereus.?Journal of Chemical Ecology 34:39-3.CrossRef PubMed
  Brucker RM, Harris RN, Schwantes CR, Gallaher TN, Flaherty DC, Lam BA, Minbiole KPC (2008b) Amphibian chemical defense: antifungal metabolites of the microsymbiont Janthinobacterium lividum on the salamander Plethodon cinereus. Journal of Chemical Ecology 34:1422-429.CrossRef PubMed
  Buchan A, LeCleir GR, Gulvik CA, González JM (2014) Masters recyclers: features and functions associated with phytoplankton blooms. Nature Reviews Microbiology 12:686-98.CrossRef PubMed
  Bustamante M, Ron S, Coloma L (2005) Cambios en la diversidad en siete comunidades de anuros en los Andes de Ecuador. Biotropica 37:180-89.CrossRef
  Cole ME, Bustamante MR, Almeida-Reinoso D, Funk WC (2014) Spatial and temporal variation in population dynamics of Andean frogs: Effects of forest disturbances and population declines. Global Ecology and Conservation 1:60-0.CrossRef
  Conlon JM, (2011) The contribution of skin antimicrobial peptides to the system of innate immunity in anurans. Cell Tissue Research 343:201-12.CrossRef PubMed
  Crawford AJ, Lips KR, Bermingham E (2010) Epidemic disease decimates amphibian abundance, species diversity, and evolutionary history in the highlands of central Panama. Proceedings of the National Academy of Sciences USA 107:13777-3782.CrossRef
  Crump ML, Scott NJ (1994) Visual encounter surveys. In: Measuring and Monitoring Biological Diversity. Standard Methods for Amphibians, Meyer W, Donnelley MA, McDiarmid RW, Hayek LAC, Foster MS (editors). Washington, DC: Smithsonian Institution Press, pp 84-2.
  Fisher MC, Henk DA, Briggs CJ, et al. (2012) Emerging fungal threats to animal, plant and ecosystem health. Nature 484:186-94.CrossRef PubMed
  Fierer N, Bradford MA, Jackson RB (2007) Toward an ecological classification of soil bacteria. Ecology 88:1354-364.CrossRef PubMed
  Flechas SV, Sarmiento C, Cárdenas ME, Medina EM, Restrepo S, Amezquita A (2012) A. Surviving chytridiomycosis: differential anti-Batrachochytrium dendrobatidis activity in bacterial isolates from three lowland species of Atelopus. Plos ONE 7(9):e44832
• 作者单位：J. C. Bresciano (1)
  C. A. Salvador (2) (3)
  C. Paz-y-Mi?o (2)
  A. M. Parody-Merino (1)
  J. Bosch (4)
  D. C. Woodhams (5) (6)
  
  1. Universidad Internacional Menendez Pelayo - Spain, Calle Arjona número 10 escalera 6 piso 4 puerta 4, Seville, Spain
  2. Instituto de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad de las Américas (UDLA), Quito, Ecuador
  3. Centro Universitario de Investigación Científica y Tecnológica, Universidad Técnica del Norte (UTN), Ibarra, Ecuador
  4. Museo Nacional de Ciencias Naturales, CSIC, José Gutiérrez Abascal 2, 28006, Madrid, Spain
  5. Department of Biology, University of Massachussets, Boston, Boston, MA, 02125, USA
  6. Smithsonian Tropical Research Institute, Balboa, Ancón, Panama, Republic of Panama
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Public Health
  Environmental Medicine/Environmental Psychology
  Ecosystems
  Environmental Management
  Microbiology
  
• 出版者：Springer New York
• ISSN：1612-9210

文摘

Amphibian populations are decreasing worldwide due to a variety of factors. In South America, the chytrid fungus Batrachochytrium dendrobatidis (Bd) is linked to many population declines. The pathogenic effect of Bd on amphibians can be inhibited by specific bacteria present on host skin. This symbiotic association allows some amphibians to resist the development of the disease chytridiomycosis. Here, we aimed (1) to determine for the first time if specific anti-Bd bacteria are present on amphibians in the Andes of Ecuador, (2) to monitor anti-Bd bacteria across developmental stages in a focal amphibian, the Andean marsupial tree frog, Gastrotheca riobambae, that deposits larvae in aquatic habitats, and (3) to compare the Bd presence associated with host assemblages including 10 species at sites ranging in biogeography from Amazonian rainforest (450 masl) to Andes montane rainforest (3200 masl). We sampled and identified skin-associated bacteria of frogs in the field using swabs and a novel methodology of aerobic counting plates, and a combination of morphological, biochemical, and molecular identification techniques. The following anti-Bd bacteria were identified and found to be shared among several hosts at high-elevation sites where Bd was present at a prevalence of 32.5%: Janthinobacterium lividum, Pseudomonas fluorescens, and Serratia sp. Bd were detected in Gastrotheca spp. and not detected in the lowlands (sites below 1000 masl). In G. riobambae, recognized Bd-resistant bacteria start to be present at the metamorphic stage. Overall bacterial abundance was significantly higher post-metamorphosis and on species sampled at lower elevations. Further metagenomic studies are needed to evaluate the roles of host identity, life-history stage, and biogeography of the microbiota and their function in disease resistance. Keywords Andes antifungal bacteria biogeography chytridiomycosis life-history microbiota