Cell type-dependent transactivation or repression of mesoderm-restricted basic helix-loop-helix protein, POD-1/Capsulin

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• 作者：Makoto Miyagishi ; Mitsutoki Hatta ; Takayuki Ohshima ; Junji Ishida ; Ryouji Fujii ; Toshihiro Nakajima and Akiyoshi Fukamizu
• 关键词：POD ; 1/Capsulin ; bHLH protein ; transcription factor ; yeast two ; hybrid system ; ITF ; 2
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2000
• 出版时间：2000
• 年：2000
• 卷：205
• 期：1/2
• 页码：141-147
• 全文大小：117 KB

文摘

A family of basic-helix-loop-helix (bHLH) nuclear factors play important roles in controlling cell growth and differentiation as critical regulatory components in transcription. Here we describe molecular characterization of mesoderm-specific bHLH protein, POD-1/Capsulin. Transactivation property of POD-1/Capsulin was analyzed by the Gal4 fusion system in six mammalian cell lines. The results indicated that an activation property was shown in HT1080 and HeLa cells, but a repression activity in HepG2 cells. Mapping analysis for the transactivation and repression activities revealed that the C-terminal domain of POD-1/Capsulin is essential for the transactivation and both the N-terminal and C-terminal domains are contributed to the repression activities. Furthermore, in order to identify possible interactants of the POD-1/Capsulin, we performed yeast two-hybrid screen in a human kidney cDNA library, and identified a class A bHLH protein, ITF-2 as potential heterodimeric partner of the bHLH protein.