Effects of garlic extract on cell wall of Corynebacterium glutamicum

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• 作者：Mouadh Mihoub (1)
  Amine Aloui (1)
  Alya EL May (1)
  Mohamed Marwan Sethom (2)
  Aouatef Ben Ammar (3)
  Mohamed Habib Jaafoura (3)
  Raoudha Kacem (1)
  Ahmed Landoulsi (1)
  
• 关键词：Corynebacterium glutamicum ; Cell wall alteration ; Garlic extract ; Electron microscopy ; Fatty acids
• 刊名：Annals of Microbiology
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：60
• 期：4
• 页码：637-643
• 全文大小：288KB
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• 作者单位：Mouadh Mihoub (1)
  Amine Aloui (1)
  Alya EL May (1)
  Mohamed Marwan Sethom (2)
  Aouatef Ben Ammar (3)
  Mohamed Habib Jaafoura (3)
  Raoudha Kacem (1)
  Ahmed Landoulsi (1)
  
  1. Unité de Biochimie des Lipides et Interaction des Macromolécules en Biologie (03/UR/0902), Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Zarzouna, 7021, Tunisia
  2. SETHOM, Laboratoire de Recherche LR99ES11, Département de Biochimie, H?pital LaRabta, Tunis, Tunisia
  3. Service Commun de Microscopie Electronique à Transmission, Faculté de Médecine de Tunis, Tunis, Tunisia
  
• ISSN：1869-2044

文摘

The Corynebacterineae represent a distinct group within Gram-positive bacteria, with prominent members being the human pathogens Mycobacterium tuberculosis, Mycobacterium leprae, and Corynebacterium diphtheriae. A common feature of the Corynebacterineae is that they possess an unusual cell wall architecture rich in mycolic acids that is responsible for host pathogenesis and antibiotic resistance. The present study was carried out to evaluate the effects of?garlic extract (GE) on the viability, cell wall ultrastructure and fatty acid composition of extractible lipids of Corynebacterium glutamicum. We found that C. glutamicum is inhibited by 44.25?mg?ml? GE. Observations by electron microscopy revealed that GE alters the cell wall. In addition, GE-treated bacteria (23.6?mg?ml?) showed an increase (from 6.9% to 18.8%) in the extractible lipids released. The fatty acid composition of these lipids was also significantly perturbed, especially C16:0, C18:0, C18:1 and C18:2. These data indicate that the integrity of the cell wall, the most effective target of antimycobacterial drugs, is affected by GE. This knowledge could contribute to improving the treatment of pathogenic Corynebacterineae.