Ozone-elicited secondary metabolites in shoot cultures of Melissa officinalis L.
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- 作者：Mariagrazia Tonelli ; Elisa Pellegrini…
- 关键词：Elicitors ; Lemon balm ; Shoot cultures ; Oxidative stress ; Phenylpropanoid pathway ; ROS
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：120
- 期：2
- 页码：617-629
- 全文大小：5,552 KB
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- 刊物主题：Life Sciences
Plant Sciences
Plant Physiology - 出版者：Springer Netherlands
- ISSN：1573-5044
文摘
The effects of ozone treatment (200?ppb, 3?h) on the accumulation of main secondary metabolites have been investigated in Melissa officinalis (lemon balm) aseptic shoot cultures in order to evaluate the biotechnological application of this gas for improving the yield of secondary metabolites of medicinal plants. During the treatment, we found (1) an activation of enzymes involved in phenolic metabolism [as confirmed by the increase of shikimate dehydrogenase, phenylalanine ammonia-lyase and cinnamyl alcohol dehydrogenase activities (about twofold higher than controls)], (2) a development of cellular barriers with a higher degree of polymerization of monolignols [as indicated by the increase of lignin (+23?% compared to controls)], (3) an accumulation of phenolic compounds, in particular rosmarinic acid (about fourfold compared to control plants cultivated in filtered air) and (4) an increase of antioxidant capacity (as documented by the improved 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity). The effect of ozone as elicitor of the production of secondary metabolites in lemon balm shoot cultures was dependent on the specific regime, the time of exposure and the concentration of the stressor. After the end of the treatment, we found cell death and hydrogen peroxide (H2O2) deposition concomitant with a prolonged superoxide anion-generation, suggesting that a transient oxidative burst had occurred.