A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS

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• 作者：Joao Benhur Mokochinski…
• 关键词：Lignin ; UHPLC ; MS ; UPLC ; MS ; S/G ratio
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：407
• 期：23
• 页码：7221-7227
• 全文大小：586 KB
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• 作者单位：Joao Benhur Mokochinski (1)
  Giovana Anceski Bataglion (2)
  Eduardo Kiyota (1)
  Letícia Marrone de Souza (1)
  Paulo Mazzafera (1)
  Alexandra Christine Helena Frankland Sawaya (1)
  
  1. Department of Plant Biology, Institute of Biology, State University of Campinas, UNICAMP, 13083-970, Campinas, S?o Paulo, Brazil
  2. ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas, UNICAMP, 13083-970, Campinas, S?o Paulo, Brazil
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

A simple extraction protocol and an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method for the determination of the syringyl/guaiacyl (S/G) ratio in lignin is reported herein. The method was entirely developed using stems of three Eucalyptus species, which were hydrolyzed with NaOH and partitioned with ethyl ether; vanillin (from the G monomer) and syringaldehyde (from S monomer) were quantified. The S/G ratios obtained were comparable to those usually reported for eucalyptus. The data for one of the eucalyptus species were compared with those obtained with a widely accepted method using thioacidolysis and gas chromatography-mass spectrometry (GC-MS). The method was also applied to sugarcane and showed to be reliable. The yield of the NaOH hydrolysis of the monolignols ranged from 89.94 to 95.69 %, with more than 77.12 % of recuperation in the liquid-liquid extraction. The whole analytical procedure was validated, achieving results with less than 4.38 % of variation. The lowest LOD and LOQ were 0.01 and 0.05 μg/mL, respectively. In addition, the method combines reliability and a fast and direct quantification.