Temperature sensing using red fluorescent protein

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• 作者：Kanagavel Deepankumar…
• 关键词：temperature sensor ; mRFP1 ; 4 ; fluoroproline ; non ; canonical amino acid incorporation ; thermal stability
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：20
• 期：1
• 页码：67-72
• 全文大小：269 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816

文摘

Genetically encoded fluorescent proteins are extensively utilized for labeling and imaging proteins, organelles, cell tissues, and whole organisms. In this study, we explored the feasibility of mRFP1 and its variants for measuring intracellular temperature. A linear relationship was observed between the temperature and fluorescence intensity of mRFP1 and its variants. Temperature sensitivities of E. coli expressing mRFP1, mRFP-P63A and mRFP-P63A[(4R)-FP] were ?.27%, ?.26% and ?.77%/°C, respectively. Finally, we demonstrated the potentiality of mRFP1 and its variants as an in vivo temperature sensor.