Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion
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- 作者：Anja Rudolf (3) (4)
Detlev Buttgereit (3)
Matthias Jacobs (3)
Georg Wolfstetter (5) (6)
D枚rthe Kesper (3) (7)
Michael P眉tz (3) (8)
Susanne Berger (3) (9)
Renate Renkawitz-Pohl (3)
Anne Holz (5)
Susanne F 脰nel (3)
3. Developmental Biology ; Department of Biology ; Philipps-Universit盲t Marburg ; Karl-von-Frisch-Stra脽e 8 ; Marburg ; 35043 ; Germany
4. INSERM U1016 Institut Cochin ; D茅partement G茅n茅tique et D茅veloppement ; 24 Rue du Faubourg Saint Jacques ; Paris ; 75014 ; France
5. Institut f眉r Allgemeine Zoologie und Entwicklungsbiologie ; Justus-Liebig-Universit盲t Gie脽en ; Stephanstra脽e 24 ; Giessen ; 35390 ; Germany
6. Department of Molecular Biology ; Building 6聽L ; Ume氓 University ; Ume氓 ; 90187 ; Sweden
7. Institute of Pathobiochemistry and Molecular Diagnostics ; Philipps-Universit盲t Marburg ; Hans-Meerwein-Stra脽e 2 ; Marburg ; 35043 ; Germany
8. Institute for Neurology ; Clinical Neurobiology ; FB20 Philipps-Universit盲t Marburg ; Baldingerstra脽e ; Marburg ; 35043 ; Germany
9. Life and Medical Sciences Institute ; Universit盲t Bonn ; Carl-Troll-Stra脽e 31 ; Bonn ; 53115 ; Germany - 关键词：Visceral musculature ; Actin regulation ; FuRMAS ; Myoblast fusion ; Site ; specific mRNA localization ; Differential transcriptional control ; Scar/Wave ; Blow ; Rols ; Kette/Nap ; 1
- 刊名：BMC Cell Biology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：15
- 期：1
- 全文大小：4,687 KB
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- 出版者：BioMed Central
- ISSN：1471-2121
文摘
Background The visceral musculature of Drosophila larvae comprises circular visceral muscles tightly interwoven with longitudinal visceral muscles. During myogenesis, the circular muscles arise by one-to-one fusion of a circular visceral founder cell (FC) with a visceral fusion-competent myoblast (FCM) from the trunk visceral mesoderm, and longitudinal muscles arise from FCs of the caudal visceral mesoderm. Longitudinal FCs migrate anteriorly under guidance of fibroblast growth factors during embryogenesis; it is proposed that they fuse with FCMs from the trunk visceral mesoderm to give rise to syncytia containing up to six nuclei. Results Using fluorescence in situ hybridization and immunochemical analyses, we investigated whether these fusion events during migration use the same molecular repertoire and cellular components as fusion-restricted myogenic adhesive structure (FuRMAS), the adhesive signaling center that mediates myoblast fusion in the somatic mesoderm. Longitudinal muscles were formed by the fusion of one FC with Sns-positive FCMs, and defects in FCM specification led to defects in longitudinal muscle formation. At the fusion sites, Duf/Kirre and the adaptor protein Rols7 accumulated in longitudinal FCs, and Blow and F-actin accumulated in FCMs. The accumulation of these four proteins at the fusion sites argues for FuRMAS-like adhesion and signaling centers. Longitudinal fusion was disturbed in rols and blow single, and scar wip double mutants. Mutants of wasp or its interaction partner wip had no defects in longitudinal fusion. Conclusions Our results indicated that all embryonic fusion events depend on the same cell-adhesion molecules, but that the need for Rols7 and regulators of F-actin distinctly differs. Rols7 was required for longitudinal visceral and somatic myoblast fusion but not for circular visceral fusion. Importantly, longitudinal fusion depended on Kette and SCAR/Wave but was independent of WASp-dependent Arp2/3 activation. Thus, the complexity of the players involved in muscle formation increases from binucleated circular muscles to longitudinal visceral muscles to somatic muscles.