Encapsulation and crystallization of Prussian blue nanoparticles by cowpea chlorotic mottle virus capsids

详细信息    查看全文

• 作者：Yuanzheng Wu (1) (2)
  Hetong Yang (2)
  Hyun-Jae Shin (1)
  
• 关键词：Biohybrid ; Bimetallic nanoparticles ; Capsids ; Cowpea chlorotic mottle virus ; Encapsulation ; Nanoparticles ; Prussian blue
• 刊名：Biotechnology Letters
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：36
• 期：3
• 页码：515-521
• 全文大小：1,689 KB
• 参考文献：1. Allison RF, Janda M, Ahlquist P (1988) Infectious in vitro transcripts from cowpea chlorotic mottle virus cDNA clones and exchange of individual RNA components with brome mosaic virus. J Virol 62:3581-588
  2. Chen Z, Stauffacher C, Johnson JE (1990) Capsid structure and RNA packaging in comovirus. Semin Virol 1:453-66
  3. Culp JT, Park JH, Frye F, Huh YD, Meisel MW, Talham DR (2005) Magnetism of metal cyanide networks assembled at interfaces. Coord Chem Rev 249:2642-648 CrossRef
  4. Daniel MC, Tsvetkova IB, Quinkert ZT, Murali A, De M, Rotello VM, Kao CC, Dragnea B (2010) Role of surface charge density in nanoparticle-templated assembly of bromovirus protein cages. ACS Nano 4:3853-860 CrossRef
  5. De la Escosura A, Verwegen M, Sikkema F, Comellas-Aragonès M, Kirilyuk A, Rasing T, Nolte RJ, Cornelissen JJ (2008) Viral capsids as templates for the production of monodisperse Prussian blue nanoparticles. Chem Commun (Camb) 13:1542-544 CrossRef
  6. De Tacconi NR, Rajeshwar K, Lezna RO (2003) Metal hexacyanoferrates: electrosynthesis, in situ characterization, and applications. Chem Mater 15:3046-062 CrossRef
  7. Dei A (2005) Photomagnetic effects in polycyanometallate compounds: an intriguing future chemically based technology? Angew Chem Int Ed 44:1160-163 CrossRef
  8. DeLongchamp DM, Hammond PT (2004) High-contrast electrochromism and controllable dissolution of assembled Prussian blue/polymer nanocomposites. Adv Funct Mater 14:224-32 CrossRef
  9. Domínguez-Vera JM, Colacio E (2003) Nanoparticles of Prussian blue ferritin: a new route for obtaining nanomaterials. Inorg Chem 42:6983-985 CrossRef
  10. Douglas T, Young M (1998) Host–guest encapsulation of materials by assembled virus protein cages. Nature 393:152-55 CrossRef
  11. Ferlay S, Mallah T, Quahe′s R, Veillet P, Verdaguer M (1995) A room-temperature organometallic magnet based on Prussian blue. Nature 378:701-03 CrossRef
  12. Fischlechner M, Donath E (2007) Viruses as building blocks for materials and devices. Angew Chem Int Ed 46:3184-193 CrossRef
  13. Sato O, Iyoda T, Fujishima A, Hashimoto K (1996) Photoinduced magnetization of a cobalt-iron cyanide. Science 272:704-05 CrossRef
  14. Sheehan D, O’Sullivan S (2003) Fast protein liquid chromatography. Protein purification protocols 244
  15. Speir JA, Munshi S, Wang G, Baker TS, Johnson JE (1995) Structures of the native and swollen forms of cowpea chlorotic mottle virus determined by X-ray crystallography and cryo-electron microscopy. Structure?3:63-8 CrossRef
  16. Taguchi M, Yamada K, Suzuki K, Sato O, Einaga Y (2005) Photoswitchable magnetic nanoparticles of Prussian blue with amphiphilic azobenzene. Chem Mater 17:4554-559 CrossRef
  17. Van der Graaf M, van Mierlo CP, Hemminga MA (1991) Solution conformation of a peptide fragment representing a proposed RNA-binding site of a viral coat protein studied by two-dimensional NMR. Biochemistry 30:5722-727 CrossRef
  18. Vriezema DM, Comellas-Aragones M, Elemans JA, Cornelissen JJ, Rowan AE, Nolte RJ (2005) Self-assembled nanoreactors. Chem Rev 105:1445-489 CrossRef
  19. Wu Y, Yang H, Shin HJ (2011) Expression and self assembly of cowpea chlorotic mottle virus capsid proteins in / Pichia pastoris and encapsulation of fluorescent myoglobin. MRS Proceedings 1317:mrsf10-1317-rr03-05 doi:10.1557/opl.2011.138
  20. Wu Y, Yang H, Shin HJ (2013) Viruses as self-assembled nanocontainers for encapsulation of functional cargoes. Korean J Chem Eng 30:1359-367 CrossRef
  
• 作者单位：Yuanzheng Wu (1) (2)
  Hetong Yang (2)
  Hyun-Jae Shin (1)
  
  1. Department of Chemical and Biochemical Engineering, Chosun University, #375 Seosuk-dong, Dong-gu, Gwangju, 501-759, Republic of Korea
  2. Biotechnology Center of Shandong Academy of Sciences, No. 19 Keyuan Road, Jinan, 250014, People’s Republic of China
  
• ISSN：1573-6776

文摘

Cowpea chlorotic mottle virus (CCMV) capsids were used to encapsulate Prussian blue (PB) particles based on electrostatic interaction. A negatively-charged metal complex, hexacyanoferrate (III), was entrapped inside the capsids through the disassembly/reassembly process under a pH change from 7.5 to 5.2. The loaded capsids reacted with a second Fe(II) to fabricate PB particles. The synthesis of PB in CCMV capsids was confirmed by a unique colour transition at 710?nm and by size-exclusion FPLC. Transmission electron microscopy images of PB-CCMV biohybrids presented discrete spherical particles with a relatively homogeneous size. Dynamic light scattering of PB-CCMV showed two peaks of 29.2?±?1.7?nm corresponding to triangulation number T?=?3 particles, and 17.5?±?1.2?nm of pseudo T?=?2 particles. The encapsulation and crystallization of PB in CCMV provided an efficient method for the self-organization of bimetallic nanoparticles.