Prussian blue nanoparticle-loaded microbubbles for photothermally enhanced gene delivery through ultrasound-targeted microbubble destruction

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• 作者：Xiaoda Li ; Xiuli Yue ; Jinrui Wang ; Xiaolong Liang ; Lijia Jing ; Li Lin…
• 关键词：Ultrasound imaging ; Microbubble ; Gene delivery ; Prussian blue nanoparticle
• 刊名：Chinese Science Bulletin
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：61
• 期：2
• 页码：148-156
• 全文大小：3,474 KB
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• 作者单位：Xiaoda Li (1)
  Xiuli Yue (1)
  Jinrui Wang (2)
  Xiaolong Liang (2)
  Lijia Jing (1)
  Li Lin (1)
  Yongbo Yang (1)
  Shanshan Feng (1)
  Yajun Qian (2)
  Zhifei Dai (2)
  
  1. School of Municipal and Environmental Engineering, School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150080, China
  2. College of Engineering, Peking University Third Hospital, Peking University, Beijing, 100871, China
  
• 刊物主题：Science, general; Life Sciences, general; Physics, general; Chemistry/Food Science, general; Earth Sciences, general; Engineering, general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-9541

文摘

By adsorbing chitosan (CS)-functionalized Prussian blue (PB) nanoparticles (CS/PB NPs) complexing DNA onto the surface of gas encapsulated microbubbles (MBs), a multifunctional gene delivery system of MBs@CS/PB/DNA was fabricated for photothermally enhanced gene transfection through ultrasound-targeted microbubble destruction. CS/PB NPs of (2.69 ± 0.49) nm could complex DNA effectively when the mass ratio was 2:1. It was found that MBs@CS/PB/DNA could enhance ultrasound imaging greatly both in vitro and in vivo. In addition, MBs@CS/PB/DNA could be disrupted by applying a higher-intensity ultrasound irradiation to release CS/PB/DNA, which could effectively transform the near-infrared (NIR) light into heat to assist the uptake of CS/PB/DNA by cells. With the aid of ultrasound irradiation and NIR light irradiation, the gene transfection efficiency was significantly enhanced to (43.08 ± 1.13) %, much higher than polyethylenimine. Moreover, MBs@CS/PB/DNA showed excellent biocompatibility, encouraging the further exploration of MBs@CS/PB/DNA to be a platform for combined ultrasound image, photothermal therapy, drug delivery, and gene therapy. Keywords Ultrasound imaging Microbubble Gene delivery Prussian blue nanoparticle