PEGylation of superparamagnetic iron oxide nanoparticle for drug delivery applications with decreased toxicity: an in vivo study
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- 作者：Suma Prabhu ; Srinivas Mutalik ; Sharada Rai…
- 关键词：Genotoxicity ; Organ ; specific toxicity ; Prussian blue staining ; Superparamagnetic iron oxide nanoparticles ; Biochemical Parameters
- 刊名：Journal of Nanoparticle Research
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：17
- 期：10
- 全文大小：7,958 KB
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Srinivas Mutalik (2)
Sharada Rai (3)
Nayanabhirama Udupa (4)
Bola Sadashiva Satish Rao (1)
1. Department of Radiation Biology & Toxicology, School of Life Sciences, Manipal University, Manipal, Karnataka, 576 104, India
2. Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, 576 104, India
3. Department of Pathology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, 575 001, India
4. Director - Research (Health Sciences), Manipal University, Manipal, Karnataka, 576104, India - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Nanotechnology
Inorganic Chemistry
Characterization and Evaluation Materials
Physical Chemistry
Applied Optics, Optoelectronics and Optical Devices - 出版者：Springer Netherlands
- ISSN：1572-896X
文摘
Superparamagnetic iron oxide nanoparticles (SPIONs) are evolving as a mainstay across various applications in the field of Science and Technology. SPIONs have enticed attention on the grounds of their unique physicochemical properties as well as potential applications in magnetic hyperthermia, immunoassays, as a contrast agent in magnetic resonance imaging and targeted drug delivery among others. Toward this goal, we synthesized SPIONs by chemical co-precipitation and PEGylated it. PEGylated SPIONs (PS) were studied for its detailed in vivo toxicity profile, in view of further surface engineering for its clinical applications. The intravenous LD50(14) of the PS was ascertained as 508.16 ± 41.52 mg/kg b wt. Histopathology of the vital organs of the animals injected with acute toxic doses showed pathological changes in spleen, lung, liver, and kidney. Accumulation of SPION was found in the aforementioned organs as confirmed by Prussian blue staining. Further, 1/10th dose of LD50(14) of PS and the Bare SPION (BS) was used to analyze a detailed toxicity profile, including genotoxicity (micronuclei formation and chromosomal aberration assays), organ-specific toxicity (a detailed serum biochemical analysis), and also determination of oxidative stress. The results of toxicity profile indicated no significant toxicity due to systemic exposure of PS. Atomic absorption spectroscopy (AAS) analysis confirmed the accumulation of SPION majorly in lungs, liver spleen, and kidneys. The present study thus indicated an optimal dose of PS which could be used for surface modification for targeted drug delivery applications with least toxicity.