Fabrication of amperometric cholesterol biosensor based on SnO₂ nanoparticles and Nafion-modified carbon paste electrode

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• 作者：Gözde Aydoğdu Tığ ; Derya Koyuncu Zeybek ; Şule Pekyardımcı
• 关键词：cholesterol biosensor ; carbon paste electrode ; amperometry ; SnO2 nanoparticles
• 刊名：Chemical Papers
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：70
• 期：6
• 页码：695-705
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry/Food Science, general; Industrial Chemistry/Chemical Engineering; Biochemistry, general; Medicinal Chemistry; Materials Science, general; Biotechnology;
• 出版者：Springer International Publishing
• ISSN：1336-9075
• 卷排序：70

文摘

This study reports the fabrication of an amperometric cholesterol biosensor based on cholesterol oxidase (ChOx), SnO₂NPs and Nafion-modified carbon paste enzyme electrodes (CPE/SnO₂NPs-ChOx/Naf). The electrochemical characterisations of BCPE and CPE/SnO₂NPs were performed using CV and EIS. The determination of cholesterol was carried out by electrochemical oxidation of H₂O₂ at 0.6 V vs. Ag/AgCl. The CPE/SnO₂NPs-ChOx/Naf presented a linear range from 0.20 μ.mol L−1 to 4.95 μmol L−1 with a low limit of detection (0.04 μ.mol L−1). In addition, the optimal values for pH and temperature were found to be 7.5 and 35°C, respectively. The CPE/SnO₂NPs-ChOx/Naf was used for the determination of cholesterol in serum samples and good results were obtained.