The vesicular nucleotide transporter (VNUT) is involved in the extracellular ATP effect on neuronal differentiation

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• 作者：Aida Menéndez-Méndez ; Juan Ignacio Díaz-Hernández…
• 关键词：VNUT ; Neuritogenesis ; Retinoic acid differentiation ; ATP ; Neuroblastoma cells ; SLC17A9
• 刊名：Purinergic Signalling
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：11
• 期：2
• 页码：239-249
• 全文大小：2,505 KB
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• 作者单位：Aida Menéndez-Méndez (1)
  Juan Ignacio Díaz-Hernández (1)
  M. Teresa Miras-Portugal (1)
  
  1. Facultad de Veterinaria, Departamento de Bioquímica y Biología Molecular IV, Universidad Complutense de Madrid, Madrid, Spain
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Pharmacology and Toxicology
  Human Physiology
  Neurosciences
  Cancer Research
  
• 出版者：Springer Netherlands
• ISSN：1573-9546

文摘

Before being released, nucleotides are stored in secretory vesicles through the vesicular nucleotide transporter (VNUT). Once released, extracellular ATP participates in neuronal differentiation processes. Thus, the expression of a functional VNUT could be an additional component of the purinergic system which regulates neuronal differentiation and axonal elongation. In vitro expression of VNUT decreases neuritogenesis in N2a cells differentiated by retinoic acid treatment, whereas silencing of VNUT expression increases the number and length of neurites in these cells. These results highlight the role of VNUT in the neuritogenic process because this transporter regulates the ATP content in neurosecretory vesicles.