Cytoskeleton involvement in lithium-induced SH-SY5Y neuritogenesis and the role of glycogen synthase kinase 3β
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- 作者：Riadh Nciri ; Mohamed Ali Boujbiha ; Samira Jbahi…
- 关键词：Lithium ; Cytoskeleton ; Neuritogenesis ; GSK3
- 刊名：Aging Clinical and Experimental Research
- 出版年：2015
- 出版时间：June 2015
- 年：2015
- 卷：27
- 期：3
- 页码：255-263
- 全文大小：3,107 KB
- 参考文献：1.Beal MF (1995) Aging, energy, and oxidative stress in neurodegenerative disorders. Ann Neurol 38:357-66View Article PubMed
2.Harman D (1998) Aging: phenomena and theories. Ann NY Acad Sci 854:1-View Article PubMed
3.Behl C (1999) Alzheimer’s disease and oxidative stress: implications for novel therapeutic approaches. Prog Neurobiol 57:301-23View Article PubMed
4.Selkoe DJ (2001) Alzheimer’s disease: genes, proteins, and therapy. Physiol Rev 81:741-66PubMed
5.Annaert W, De Strooper B (2002) A cell biological perspective on Alzheimer’s disease. Annu Rev Cell Dev Biol 18:25-1View Article PubMed
6.Riadh Nciri, Allagui Mohamed Salah, Bourogaa Ezzedine, Vincent Christian, Croute Fran?oise, Elfeki Abdelfattah (2011) Neuroprotective and neurotrophic effects of long term lithium treatment in mouse brain. Biometals 24:747-57View Article PubMed
7.Quiroz JA, Machado-Vieira R, Zarate CA, Manji JHK (2010) Novel insights into lithium’s mechanism of action: neurotrophic and neuroprotective effects. Neuropsychobiology 62:50-0View Article PubMed Central PubMed
8.Italia J, Mukhopadhyaya R, Rajadhyaksha MS (2011) Differential display RT-PCR reveals genes associated with lithium-induced neuritogenesis in SK-N-MC cells. Cell Mol Neurobiol 31:1021-026View Article PubMed
9.Mora A, Gonzalez-Polo RA, Fuentes JM, Soler G, Centeno F (1999) Different mechanisms of protection against apoptosis by valproate and Li+. Eur J Biochem 266:886-91View Article PubMed
10.Bauer M, Alda M, Priller J, Young LT (2003) International Group for the Study of Lithium Treated Patients (IGSLI). Implications of the neuroprotective effects of lithium for the treatment of bipolar and neurodegenerative disorders. Pharmacopsychiatry 36:250-54View Article
11.Chuang DM (2004) Neuroprotective and neurotrophic actions of the mood stabilizer lithium: can it be used to treat neurodegenerative diseases. Crit Rev Neurobiol 16:83-0View Article PubMed
12.Jin N, Kovacs AD, Sui Z, Dewhurst S, Maggirwar SB (2005) Opposite effects of lithium and valproic acid on trophic factor deprivation-induced glycogen synthase kinase-3 activation, c-Jun expression and neuronal cell death. Neuropharmacology 48:576-83View Article PubMed
13.Berridge MJ, Downes CP, Hanley MR (1989) Neural and developmental actions of lithium: a unifying hypothesis. Cell 59:411-19View Article PubMed
14.Klein PS, Melton DA (1996) A molecular mechanism for the effect of lithium on development. Proc Natl Acad Sci USA 93:8455-459View Article PubMed Central PubMed
15.Stambolic V, Ruel L, Woodgett JR (1996) Lithium inhibits glycogen synthase kinase-3 activity and mimics wingless signalling in intact cells. Curr Biol 6:1664-668View Article PubMed
16.Phiel CJ, Klein PS (2001) Molecular targets of lithium action. Annu Rev Pharmacol Toxicol 41:789-13View Article PubMed
17.Grimes CA, Jope RS (2001) The multifaceted roles of glycogen synthase kinase 3beta in cellular signaling. Prog Neurobiol 65:391-26View Article PubMed
18.Jope RS (2003) Lithium and GSK-3: one inhibitor, two inhibitory actions, multiple outcomes. Trends Pharmacol Sci 24:441-43View Article PubMed
19.Sasaki T, Han F, Shioda N, Moriguchi S, Kasahara J, Ishiguro K, Fukunaga K (2006) Lithium-induced activation of Akt and CaM kinase II contributes to its neuroprotective action in a rat microsphere embolism model. Brain Res 1108:98-06View Article PubMed
20.Liang MH, Wendland JR, Chuang DM (2008) Lithium inhibits Smad3/4 transactivation via increased CREB activity induced by enhanced PKA and AKT signaling. Mol Cell Neurosci 37:440-53View Article PubMed Central PubMed
21.Bielecka AM, Obuchowicz E (2008) Antiapoptotic action of lithium and valproate. Pharmacol Rep 60:771-82PubMed
22.Marmol F (2008) Lithium: bipolar disorder and neurodegenerative diseases, possible cellular mechanisms of the therapeutic effects of lithium. Prog Neuropsychopharmacol Biol Psychiatry 32:1761-771View Article PubMed
23.Machado-Vieira R, Manji HK, Zarate CA (2009) The role of lithium in the treatment of bipolar disorder: convergent evidence for neurotrophic effects as a unifying hypothesis. Bipolar Disord 11:92-09View Article PubMed Central PubMed
24.Wada A (2009) Lithium and neuropsychiatric therapeutics: neuroplasticity via glycogen synthase kinase-3beta, beta-catenin, and neurotrophin cascades. J Pharmacol Sci 110:14-8View Article PubMed
25.Lowry OH, Rosebrouch NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-75PubMed
26.Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-85View Article PubMed
27.Jope RS (1999) Anti-bipolar therapy: mechanism of action of lithium. Mol Psych 4:117-28View Article
28.Burgoyne RD (1991) High molecular weight microtubule-associated proteins of brain. In: Burgoyne RD (ed) The neuronal cytoskeleton. Wiley, - 作者单位：Riadh Nciri (1) (2)
Mohamed Ali Boujbiha (2)
Samira Jbahi (2)
Mohamed Salah Allagui (1) (2)
Abdelfattah Elfeki (2)
Christian Vincent (1)
Fran?oise Croute (1)
1. Laboratoire de Biologie Cellulaire, Faculté de Médecine Purpan, Universite Paul Sabatier Toulouse III, 37 Allées Jules Guesde, Toulouse, France
2. Laboratoire d’Ecophysiologie Animale, Faculté des Sciences de Sfax, PB 802, 3018, Sfax, Tunisia - 刊物主题：Geriatrics/Gerontology;
- 出版者：Springer International Publishing
- ISSN：1720-8319
文摘
Lithium modulates signals impacting on the cytoskeleton, a dynamic system contributing to neural plasticity at multiple levels. In this study, SH-SY5Y human neuronal cells were cultured in the absence (C) or in presence (Li) of a 0.5?mM Li2CO3 (i.e. 1?mM lithium ion) for 25-0?weeks. We investigated the effect of this treatment on (1) morphological changes of cells observed using Hemalun eosin staining assay, (2) cytoskeletal changes by indirect immunofluorescence (IIF) staining of microtubules (α-tubulin) and heavy neurofilaments subunits (NF-H) and by measuring the expression rate changes of genes coding for receptor for activated C kinase (RACK1), casein kinase2 (CK2) and thymosine beta-10 using cDNA arrays technology, (3) cell adhesion properties by IIF staining of β-catenin protein. Besides, we have tried to understand the molecular mechanism of lithium action that triggers changes in cytoskeleton and neurites outgrowth. Thus, we examined the effect of this treatment on glycogen synthase kinase 3 (GSK3) expression and activity using western blotting of GSK3 and phosphorylated β-catenin, a downstream GSK3 target protein. Our results showed that lithium treatment reduces axon length, increases axonal spreading, enhances neurites growth and neurites branching with an increase of growth cone size. Moreover, genes coding for CK2 and thymosine beta-10 were significantly up-regulated, however, that coding for RACK1 was down-regulated. The most interesting result in this work is that mechanism underlying lithium action was not related to the inhibition of GSK3 activity. In fact, neither expression rate nor activity of this protein was changed.