Development and Evaluation of Artesunate-Loaded Chitosan-Coated Lipid Nanocapsule as a Potential Drug Delivery System Against Breast Cancer

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• 作者：Tuan Hiep Tran ; Tuan Duc Nguyen ; Bijay Kumar Poudel ; Hanh Thuy Nguyen…
• 关键词：anti ; cancer ; artesunate ; breast cancer ; chitosan ; lipid nanoparticles
• 刊名：AAPS PharmSciTech
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：16
• 期：6
• 页码：1307-1316
• 全文大小：4,165 KB
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• 作者单位：Tuan Hiep Tran (2)
  Tuan Duc Nguyen (1)
  Bijay Kumar Poudel (2)
  Hanh Thuy Nguyen (1)
  Jong Oh Kim (2)
  Chul Soon Yong (2)
  Chien Ngoc Nguyen (1)
  
  2. College of Pharmacy, Yeungnam University, 214-1, Dae-Dong, Gyeongsan, 712-749, South Korea
  1. National Institute of Pharmaceutical Technology, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Hanoi, Vietnam
  
• 刊物主题：Pharmacology/Toxicology; Biotechnology; Biochemistry, general; Pharmacy;
• 出版者：Springer US
• ISSN：1530-9932

文摘

Artesunate (ART)—a well-known hydrophobic anti-malarial agent was incorporated in a polymer-lipid hybrid nanocolloidal system for anti-cancer therapeutic. The lipid negatively charged nanoemulsion was formulated by modified hot homogenization method then covered with positively charged chitosan via electrostatic interaction to obtain chitosan-coated lipid nanocapsule (ART-CLN). Physical properties of the system were characterized in terms of size, charge, morphology, drug loading capacity, and physical state. In addition, anti-cancer activities were confirmed by conducting MTT assay for ART and ART-CLN on different cancer cell lines. Obtained ART-CLN after coating chitosan revealed positive charge (13.2?±-.87 mV), small particle size (160.9?±-.5 nm), and spherical shape. High drug entrapment efficiency (95.49?±-.13%) and sustained release pattern were observed. Moreover, the good cellular uptake was recorded by flow cytometry as well as confocal image. Finally, ART-CLN exhibited stronger anti-cancer activity than free ART on breast cancer cell lines (MCF-7, MDA-MB-231). These results suggested that by loading ART into lipid core of polymer-lipid hybrid carrier, the activity and physical stability of ART can be significantly increased for cancer chemotherapy. KEY WORDS anti-cancer artesunate breast cancer chitosan lipid nanoparticles