Screening and structure-based modeling of T-cell epitopes of Nipah virus proteome: an immunoinformatic approach for designing peptide-based vaccine

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• 作者：Mohit Kamthania ; D. K. Sharma
• 关键词：Nipah virus ; Molecular modeling ; T ; cell epitope ; Vaccine designing ; MHC class I alleles
• 刊名：3 Biotech
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：5
• 期：6
• 页码：877-882
• 全文大小：828 KB
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• 作者单位：Mohit Kamthania (1) (2)
  D. K. Sharma (3)
  
  1. Mangalayatan University, Aligarh-Mathura Highway, Beswan, Aligarh, Uttar Pradesh, India
  2. Jiwaji University, Gwalior, Madhya Pradesh, India
  3. Department of Zoology, Government Post Graduate College, Guna, Madhya Pradesh, India
  
• 刊物主题：Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-5738

文摘

Identification of Nipah virus (NiV) T-cell-specific antigen is urgently needed for appropriate diagnostic and vaccination. In the present study, prediction and modeling of T-cell epitopes of Nipah virus antigenic proteins nucleocapsid, phosphoprotein, matrix, fusion, glycoprotein, L protein, W protein, V protein and C protein followed by the binding simulation studies of predicted highest binding scorers with their corresponding MHC class I alleles were done. Immunoinformatic tool ProPred1 was used to predict the promiscuous MHC class I epitopes of viral antigenic proteins. The molecular modelings of the epitopes were done by PEPstr server. And alleles structure were predicted by MODELLER 9.10. Molecular dynamics (MD) simulation studies were performed through the NAMD graphical user interface embedded in visual molecular dynamics. Epitopes VPATNSPEL, NPTAVPFTL and LLFVFGPNL of Nucleocapsid, V protein and Fusion protein have considerable binding energy and score with HLA-B7, HLA-B*2705 and HLA-A2MHC class I allele, respectively. These three predicted peptides are highly potential to induce T-cell-mediated immune response and are expected to be useful in designing epitope-based vaccines against Nipah virus after further testing by wet laboratory studies. Keywords Nipah virus Molecular modeling T-cell epitope Vaccine designing MHC class I alleles