Eukaryotic elongation factor 1-beta interacts with the 5′ untranslated region of the M gene of Nipah virus to promote mRNA translation

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• 作者：Shotaro Uchida ; Hiroki Sato ; Misako Yoneda ; Chieko Kai
• 刊名：Archives of Virology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：161
• 期：9
• 页码：2361-2368
• 全文大小：1,236 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798
• 卷排序：161

文摘

Nipah virus belongs to the genus Henipavirus in the family Paramyxoviridae, and its RNA genome is larger than those of other paramyxoviruses because it has long untranslated regions (UTRs) in each gene. However, the functions of these UTRs are not fully understood. In this study, we investigated the functions of the 5′ UTRs and found that the 5′ UTR of the M gene upregulated the translation of a reporter gene. Using an RNA pull-down assay, we showed that eukaryotic elongation factor 1-beta (EEF1B2) interacts with nucleotides 81–100 of the M 5′ UTR and specifically enhances its translation efficiency. Our results suggest that the M 5′ UTR promotes the production of M protein and viral budding by recruiting EEF1B2.