Toxicological interactions between the mycotoxins deoxynivalenol, nivalenol and their acetylated derivatives in intestinal epithelial cells

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• 作者：Imourana Alassane-Kpembi ; Olivier Puel ; Isabelle P. Oswald
• 关键词：Combination index ; Cytotoxicity ; Non ; transformed intestinal cell ; Mycotoxin ; Synergy ; Trichothecenes
• 刊名：Archives of Toxicology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：89
• 期：8
• 页码：1337-1346
• 全文大小：551 KB
• 参考文献：Alassane-Kpembi I, Kolf-Clauw M, Gauthier T, Abrami R, Abiola FA, Oswald IP, Puel O (2013) New insights into mycotoxin mixtures: the toxicity of low doses of Type B trichothecenes on intestinal epithelial cells is synergistic. Toxicol Appl Pharmacol 272:191-98PubMed View Article
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• 作者单位：Imourana Alassane-Kpembi (1) (2) (3)
  Olivier Puel (1) (2)
  Isabelle P. Oswald (1) (2)
  
  1. UMR 1331 Toxalim, Research Center in Food Toxicology, INRA, 180 chemin de Tournefeuille, BP93173, 31027, Toulouse cedex 03, France
  2. INP, UMR 1331, Toxalim, Université de Toulouse, 31076, Toulouse, France
  3. H?pital d’Instruction des Armées, Camp Guézo, 01BP517, Cotonou, Bénin
  
• 刊物主题：Pharmacology/Toxicology; Occupational Medicine/Industrial Medicine; Environmental Health; Biomedicine general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1432-0738

文摘

In case of mycotoxin contaminations, food and feedstuff are usually contaminated by more than one toxin. However toxicological data concerning the effects of mycotoxin combinations are sparse. The intestinal epithelium is the first barrier against food contaminants and this constantly renewing organ is particularly sensitive to mycotoxins. The aim of this study was to investigate the effects of deoxynivalenol (DON) and four other type B trichothecenes (TCTB), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), nivalenol (NIV) and fusarenon-X (FX) alone or in combination on intestinal epithelial cells. Proliferating, non-transformed IPEC-1 cells were exposed to increasing doses of TCTB, alone or in binary mixtures and mycotoxin-induced cytotoxicity was measured with MTT test. The toxicological interactions were assessed using the isobologram-Combination index method. The five tested mycotoxins and their mixtures had a dose-dependent effect on the proliferating enterocytes. DON–NIV, DON-5-ADON and 15-ADON--ADON combinations were synergistic, with magnitude of synergy for 10?% cytotoxicity ranging from 2 to 7. The association between DON and 3-ADON also demonstrated a synergy but only at high doses, at lower doses antagonism was noted. Additivity was observed between NIV and FX, and antagonism between DON and FX. These results indicate that the simultaneous presence of mycotoxins in food commodities and diet may be more toxic than predicted from the mycotoxins alone. This synergy should be taken into account considering the frequent co-occurrence of TCTB in the diet.