Genetic variation and evolution of the Pit blast resistance locus in rice

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• 作者：Jinbin Li (1)
  Yiding Sun (2)
  Hui Liu (3) (4)
  Yanyan Wang (2)
  Yulin Jia (5)
  Minghui Xu (2)
  
• 关键词：Blast disease ; Evolution ; Magnaporthe oryzae ; Oryza sativa ; Pit ; Resistance gene
• 刊名：Genetic Resources and Crop Evolution
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：61
• 期：2
• 页码：473-489
• 全文大小：526 KB
• 作者单位：Jinbin Li (1)
  Yiding Sun (2)
  Hui Liu (3) (4)
  Yanyan Wang (2)
  Yulin Jia (5)
  Minghui Xu (2)
  
  1. Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences (YAAS), Kunming, 650205, Yunnan Province, China
  2. The Key Laboratory of Biotechnology Research of Yunnan Province, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences (YAAS), Kunming, 650223, Yunnan Province, China
  3. Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, Yunnan Province, China
  4. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China
  5. United States Department of Agriculture–Agriculture Research Service, Dale Bumpers National Rice Research Center, Stuttgart, AR, USA
  
• ISSN：1573-5109

文摘

Resistance (R) gene Pit in rice, encodes a protein with nucleotide binding sites–leucine rich repeat domain, prevents infections by strains of Magnaporthe oryzae in a gene for gene manner. Here, we analyzed the open reading frame (ORF) of Pit in 26 varieties including Oryza sativa L. subsp. indica Kato (Aus), indica (IND), temperate japonica (TEJ), tropical japonica (TRJ), aromatic (ARO) subgroups of Oryza sativa, and nine accessions of wild rice relatives including O. nivara and O. rufipogon from different growing areas. Twenty-one nucleotide differences were found within the ORFs of 35 accessions. Translation of these ORFs revealed a total of 11 Pit variants, seven of which were novel. Furthermore, variant H1 was identified in O. sativa and O. nivara; H2 and H10 were identified in O. sativa and O. rufipogon. H3, H4, and H11 were only found in O. sativa. H11 was the common type of TEJ and ARO; H3 and H4 was the specific type of TRJ and IND, respectively. H5, H6, and H7 were specific haplotypes of O. nivara, while, H8 and H9 were specific haplotypes of O. rufipogon. These results suggest that Pit is an ancient gene that exists before formation of rice domestication subgroups.