Electroretinographic assessment of retinal function during acute exposure to normobaric hypoxia

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• 作者：Andreas Schatz (1)
  Maurice Breithaupt (1)
  Jens Hudemann (2)
  Andreas Niess (2)
  André Messias (3)
  Eberhart Zrenner (1)
  Karl Ulrich Bartz-Schmidt (1)
  Florian Gekeler (1)
  Gabriel Willmann (1)
  
• 关键词：Electroretinography ; Retinal function ; Hypoxia ; Normobaric hypoxia ; Simulated hypoxia
• 刊名：Graefe's Archive for Clinical and Experimental Ophthalmology
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：252
• 期：1
• 页码：43-50
• 全文大小：327 KB
• 作者单位：Andreas Schatz (1)
  Maurice Breithaupt (1)
  Jens Hudemann (2)
  Andreas Niess (2)
  André Messias (3)
  Eberhart Zrenner (1)
  Karl Ulrich Bartz-Schmidt (1)
  Florian Gekeler (1)
  Gabriel Willmann (1)
  
  1. Centre for Ophthalmology, University of Tübingen, Schleichstr. 12-16, 72076, Tübingen, Germany
  2. Medical Clinic and Polyclinic, Department of Sports Medicine, University of Tübingen, Tübingen, Germany
  3. Department of Ophthalmology, Otorhinolaryngology, and Head and Neck Surgery, School of Medicine of Ribeir?o Preto -University of S?o Paulo, S?o Paulo, Brazil
  
• ISSN：1435-702X

文摘

Background The current study aimed to investigate retinal function during exposure to normobaric hypoxia. Methods Standard Ganzfeld ERG equipment (Diagnosys LLC, Cambridge, UK) using an extended ISCEV protocol was applied to explore intensity–response relationship in dark- and light- adapted conditions in 13 healthy volunteers (mean age 25?±-?years). Baseline examinations were performed under atmospheric air conditions at 341?meters above sea level (FIO2 of 21?%), and were compared to hypoxia (FIO2 of 13.2?%) by breathing a nitrogen-enriched gas mixture for 45?min. All subjects were monitored using infrared oximetry and blood gas analysis. Results The levels of PaCO2 changed from 38.4?±-.7?mmHg to 36.4?±-.0?mmHg, PaO2 from 95.5?±-.9?mmHg to 83.7?±-.6?mmHg, and SpO2 from 100?±-?% to 87?±-?%, from baseline to hypoxia respectively. A significant decrease (p-lt;-.05) was found for saturation amplitude of the dark-adapted b-wave intensity–response function (Vmax), dark-adapted a- and b-wave amplitudes of combined rod and cone responses (3 and 10?cd.s/m2), light-adapted b-wave amplitudes of single flash (3 and 10?cd.s/m2), and flicker responses (5-5?Hz) during hypoxia compared to baseline, without changes in implicit times. The a-wave slope of combined rod and cone responses (3 and 10?cd.s/m2) and the oscillatory potentials were significantly lower during hypoxia (p-lt;-.05). A isolated light-adapted ON response (250?ms flash) showed a reduction of amplitudes at hypoxia (p-lt;-.05), but no changes were observed for the OFF response. Conclusions The results show significant impairment of retinal function during simulated normobaric short-term hypoxia affecting specific retinal cells of rod and cone pathways.