New record on karyological analysis and first study of NOR localization of parthenogenetic brahminy blind snake, Ramphotyphlops braminus (Squamata, Typhlopidae) in Thailand

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• 作者：Isara Patawang ; A. Tanomtong ; P. Kaewmad ; Y. Chuaynkern ; P. Duengkae
• 关键词：Ramphotyphlops braminus ; Karyotype ; Chromosome
• 刊名：The Nucleus
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：59
• 期：1
• 页码：61-66
• 全文大小：589 KB
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• 作者单位：Isara Patawang (1)
  A. Tanomtong (1)
  P. Kaewmad (2)
  Y. Chuaynkern (1)
  P. Duengkae (3)
  
  1. Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen, 40002, Thailand
  2. Major of Biology, Faculty of Science and Technology, Mahasarakham Rajabhat University, Muang, Mahasarakham, 44000, Thailand
  3. Department of Forest Biology, Faculty of Forestry, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand
  
• 刊物主题：Cell Biology;
• 出版者：Springer India
• ISSN：0976-7975

文摘

This is chromosomal analysis of the brahminy blind snake, Ramphotyphlops braminus (Daudin, 1803), a newly recorded species in northeastern Thailand. Mitotic chromosomes were prepared directly from intestinal epithelia cells of five brahminy blind snakes. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results showed that the triploid chromosome number was 3n = 42, while the fundamental number (NF) was 78. The types of chromosomes were 24 macrochromosomes (19 m + 5sm) and 18 microchromosomes (9 m + 3sm + 6 t). The region adjacent to the short arm near the telomere of one in macrochromosome pair 8 showed a clearly observable nucleolar organizer region/NOR. There were no irregularly sized chromosomes related to sex. The karyotype formula for R. braminus is as follows: 3n (42) = Lm ₁₂ + Sm ₇ + Ssm ₅ + 18 microchromosomes.